Evidence map›Paper›PMID 34264456›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants.

Richard E Wiemels, Rebecca A Keogh, Ronan K Carroll

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Richard E WiemelsDepartment of Biological Sciences, Ohio University, Athens, OH, USA.
Rebecca A KeoghDepartment of Biological Sciences, Ohio University, Athens, OH, USA.
Ronan K CarrollDepartment of Biological Sciences, Ohio University, Athens, OH, USA. carrolr3@ohio.edu.
Ohio University · US

Funding

Characterizing the role of PrsB, an intracellular PPIase that is required for activity of secreted virulence factors in Staphylococcus aureusR21AI128376 · NIAID · OHIO UNIVERSITY ATHENS · PI CARROLL, RONAN · 2017 to 2018
$415k
NIAID NIH HHS R21 AI128376
6 · The paper itself

Abstract

Staphylococcal secreted nuclease contributes to S. aureus virulence by degrading neutrophil extracellular traps (NETs), which allows the bacterium to evade the host immune system and has also been shown to promote biofilm dispersal. In this chapter, two methods for detecting nuclease activity are described, both of which have increased sensitivity compared to the traditional nuclease agar method.

Indexed as

AnimalsBacterial ProteinsBacteriological TechniquesExtracellular TrapsImmune EvasionMaleMicrococcal NucleaseSalmonSpermatozoaStaphylococcus aureusVirulence FactorsBacterial ProteinsMicrococcal Nucleasenuc protein, staphylococcusVirulence FactorsFRETNucleaseSalmon sperm DNAStaphylococcusVirulence factor

Identifiers

PMID34264456
PMCPMC10152981
OpenAlexW3180704441

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.