Evidence map›Paper›PMID 34263031›Full record

ArticleAPL photonics2021

Label-free image-encoded microfluidic cell sorter with a scanning Bessel beam.

Xinyu Chen, Lauren Waller, Jiajie Chen, Rui Tang, Zunming Zhang, Ivan Gagne, Bien Gutierrez, Sung Hwan Cho, Chi-Yang Tseng, Ian Y Lian and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in APL photonics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. A high-throughput technique to map cell images to cell positions using a 3D imaging flow cytometer.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinyu ChenDepartment of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.ORCID 0000-0002-9507-5835
Lauren WallerDepartment of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.ORCID 0000-0003-4778-6082
Jiajie ChenCollege of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen 518060, China.ORCID 0000-0002-7200-6626
Rui TangDepartment of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.ORCID 0000-0001-7024-3831
Zunming ZhangDepartment of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.
Ivan GagneNanoCellect Biomedical, Inc., San Diego, California 92121, USA.
Bien GutierrezNanoCellect Biomedical, Inc., San Diego, California 92121, USA.
Sung Hwan ChoNanoCellect Biomedical, Inc., San Diego, California 92121, USA.
Chi-Yang TsengDepartment of Materials Science and Engineering, University of California, San Diego, La Jolla, California 92093, USA.
Ian Y LianDepartment of Biology, Lamar University, Beaumont, Texas 77710, USA.
Yu-Hwa LoDepartment of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92093, USA.ORCID 0000-0002-4102-4602

Funding

3D-FACS: 3D image-based fluorescence activated cell sortingR44DA045460 · NIDA · NANOCELLECT BIOMEDICAL, INC. · PI CHO, SUNG HWAN, LO, YU-HWA · 2019 to 2020
$1.5M
NIDA NIH HHS R44 DA045460
6 · The paper itself

Abstract

The microfluidic-based, label-free image-guided cell sorter offers a low-cost, high information content, and disposable solution that overcomes many limitations in conventional cell sorters. However, flow confinement for most microfluidic devices is generally only one-dimensional using sheath flow. As a result, the equilibrium distribution of cells spreads beyond the focal plane of commonly used Gaussian laser excitation beams, resulting in a large number of blurred images that hinder subsequent cell sorting based on cell image features. To address this issue, we present a Bessel-Gaussian beam image-guided cell sorter with an ultra-long depth of focus, enabling focused images of >85% of passing cells. This system features label-free sorting capabilities based on features extracted from the output temporal waveform of a photomultiplier tube (PMT) detector. For the sorting of polystyrene beads, SKNO1 leukemia cells, and

Identifiers

PMID34263031
PMCPMC8259130

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.