ArticleJournal of tissue engineering
Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Biochemical and Physicomechanical Cues of Biomaterials Guide Osteogenic Differentiation of Mesenchymal Stem Cells.International journal of molecular sciences · 2026Review
- Nanoemulgel Development of Stem Cells from Human Exfoliated Deciduous Teeth-Derived Conditioned Medium as a Novel Nanocarrier Growth Factors.European journal of dentistry · 2026Article
- Hyaluronic acid-coated Poly(L-lactide-co-1,3-trimethylene carbonate) modulate early cellular-scaffold interactions and osteogenic potential: a comprehensiveFrontiers in bioengineering and biotechnology · 2026Article
- Injectable Nanocomposite Biomaterial for 3D Printing of Personalized Matrices and Their Use in Bioreactors for Bioengineering Advanced Cell Culture Models.ACS applied materials & interfaces · 2025Article
- Optogenetic activation of mechanical nociceptions to enhance implant osseointegration.Nature communications · 2025Article
- Correlation between Ca Release and Osteoconduction by 3D-Printed Hydroxyapatite-Based Templates.ACS applied materials & interfaces · 2024Article
- Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds.Biotechnology letters · 2023Article
- Effectiveness of the production of tissue-engineered living bone graft: a comparative study using perfusion and rotating bioreactor systems.Scientific reports · 2023Article
- Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.Bioengineering & translational medicine · 2023Article
- Integrating physicomechanical and biological strategies for BTE: biomaterials-induced osteogenic differentiation of MSCs.Theranostics · 2023Review
- Article
- Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering.Computational and structural biotechnology journal · 2023Review
- Contact osteogenesis by biodegradable 3D-printed poly(lactide-co-trimethylene carbonate).Biomaterials research · 2022Article
- Premixed Calcium Silicate-Based Root Canal Sealer Reinforced with Bioactive Glass Nanoparticles to Improve Biological Properties.Pharmaceutics · 2022Article
- Article
- Efficacy of treating segmental bone defects through endochondral ossification: 3D printed designs and bone metabolic activities.Materials today. Bio · 2022Article
- Bioengineered Living Bone Grafts-A Concise Review on Bioreactors and Production Techniques In Vitro.International journal of molecular sciences · 2022Review
- Optimization and Validation of a Custom-Designed Perfusion Bioreactor for Bone Tissue Engineering: Flow Assessment and Optimal Culture Environmental Conditions.Frontiers in bioengineering and biotechnology · 2022Article
- An optimized 3D-printed perfusion bioreactor for homogeneous cell seeding in bone substitute scaffolds for future chairside applications.Scientific reports · 2021Article
- Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell-Extracellular Matrix Interactions.Materials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fatal determination of bone marrow mesenchymal stem/stromal cells (BMSC) is closely associated with mechano-environmental factors in addition to biochemical clues. The aim of this study was to induce osteogenesis in the absence of chemical stimuli using a custom-designed laminar flow bioreactor. BMSC were seeded onto synthetic microporous scaffolds and subjected to the subphysiological level of fluid flow for up to 21 days. During the perfusion, cell proliferation was significantly inhibited. There were also morphological changes, with F-actin polymerisation and upregulation of ROCK1. Notably, in BMSC subjected to flow, mRNA expression of osteogenic markers was significantly upregulated and RUNX2 was localised in the nuclei. Further, under perfusion, there was greater deposition of collagen type 1 and calcium onto the scaffolds. The results confirm that an appropriate level of fluid stimuli preconditions BMSC towards the osteoblastic lineage on 3D scaffolds in the absence of chemical stimulation, which highlights the utility of flow bioreactors in bone tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.