Evidence map›Paper›PMID 34262087›Full record

ArticleScientific reports2021

Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans.

Tao Zhang, Cheng Ni, Cheng Li, Pan Lu, Dan Chen, Yuanlin Dong, Johnathan R Whetstine, Yiying Zhang, Zhongcong Xie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Tao Zhang *Department of Anesthesiology, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
Cheng Ni *Department of Anesthesiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.
Cheng Li *Department of Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, 200434, China.
Pan LuGeriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Room 4310, Charlestown, MA, 02129-2060, USA.
Dan ChenDepartment of Anesthesiology, Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
Yuanlin DongGeriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Room 4310, Charlestown, MA, 02129-2060, USA.
Johnathan R WhetstineCancer Epigenetics Institute, Fox Chase Cancer Center, Philadelphia, PA, 19111, USA.
Yiying ZhangGeriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Room 4310, Charlestown, MA, 02129-2060, USA. yzhang37@mgh.harvard.edu.
Zhongcong XieGeriatric Anesthesia Research Unit, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Room 4310, Charlestown, MA, 02129-2060, USA. zxie@mgh.harvard.edu.
Massachusetts General Hospital · USHarvard University · USChinese Academy of Medical Sciences & Peking Union Medical College · CNFox Chase Cancer Center · USShanghai Tenth People's Hospital · CN

Funding

WORD PROCESSING CENTER--COREP30CA006927 · NCI · RESEARCH INST OF FOX CHASE CAN CTR · PI Margaret von Mehren · 1985 to 2026
$138.8M
General Anesthesia and Alzheimer's Disease NeuropathogenesisR01GM088801 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI XIE, ZHONGCONG · 2009 to 2017
$3.1M
Tau/P-Tau as Biomarkers of Anesthesia- and Surgery-Induced Cognitive Impairment in a Murine ModelR01HD086977 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI XIE, ZHONGCONG · 2016 to 2020
$1.8M
NCI NIH HHS P30 CA006927NICHD NIH HHS R01 HD086977NIGMS NIH HHS R01 GM088801NIH HHS R01GM088801
6 · The paper itself

Abstract

Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.

Indexed as

Anesthetics, InhalationAnimalsApoptosisCaenorhabditis elegansCaenorhabditis elegans ProteinsCaspasesEmbryo, NonmammalianFemaleHermaphroditic OrganismsIsofluraneMaleOogenesisOxidative StressProto-Oncogene Proteins c-ablReactive Oxygen SpeciesTumor Suppressor Protein p53ABL-1 protein, C elegansAnesthetics, InhalationCaenorhabditis elegans ProteinsCaspasesced-3 protein, C elegansCEP-1 protein, C elegansIsofluraneProto-Oncogene Proteins c-ablReactive Oxygen SpeciesTumor Suppressor Protein p53

Identifiers

PMID34262087
PMCPMC8280124
OpenAlexW3178717982

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.