Evidence map›Paper›PMID 34261981›Full record

ArticleJournal of the Chinese Medical Association : JCMA2021

Roles and mechanisms of circulating CEACAM1 in the cirrhosis-related intestinal hyperpermeability: in vitro approach.

Chien-Fu Hsu, Ming-Wei Lin, Chia-Chang Huang, Tzu-Hao Li, Chih-Wei Liu, Shiang-Fen Huang, Ying-Ying Yang, Yi-Hsiang Huang, Ming-Chih Hou, Han-Chieh Lin

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In one paragraph

Article in Journal of the Chinese Medical Association : JCMA, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Chien-Fu HsuDepartment of Medical Education, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Ming-Wei LinFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
Chia-Chang HuangDepartment of Medical Education, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Tzu-Hao LiDivision of Preventive Medicine, Institute of Public Health, National Yang Ming Chiao Tung University, Taipei, Taiwan, ROC.
Chih-Wei LiuFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
Shiang-Fen HuangFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
Ying-Ying YangDepartment of Medical Education, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Yi-Hsiang HuangFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
Ming-Chih HouFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
Han-Chieh LinFaculty of Medicine, National Yang Ming Chiao Tung Universityl, Taipei, Taiwan, ROC.
National Yang Ming Chiao Tung University · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCirrhosis-related intestinal hyperpermeability and endotoxemia are characterized by intestinal epithelial cell apoptosis, impaired restitution (proliferation and migration), decreased tight junction protein levels, and subsequent barrier dysfunction. In addition to endotoxin and tumor necrosis factor-α (TNFα), carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) plays crucial roles in the regulation of apoptosis, restitution, tight junction protein-maintained barrier function of intestinal epithelial cells.

methodsThis study aims to explore the roles and underlying mechanisms of CEACAM1 in cirrhosis-related intestinal hyperpermeability through in vitro approach.

resultsIn cirrhotic patients, high serum levels of intestinal hyperpermeability (zonulin and endotoxin) markers were accompanied by elevated serum levels of TNFα and soluble CEACAM1. In in vitro experiments, we evaluated the individual and interacted roles of TNFα and human recombinant CEACAM1 (hrCEACAM1) in LC-sera (sera of cirrhotic patients)-induced intestinal hyperpermeability-related pathogenic signals. In the cell Line human from human colon (Caucasian colon adenocarcinoma) (Caco-2) cell culture, LC-sera, TNFα, and hrCEACAM1 increased apoptosis (measured by Terminal deoxynucleotidyl transferase [TdT] dUTP nick end labeling+/annexin-5+propidium iodide+ cells and caspase-3 activity), decreased restitution capacity (proliferation and migration), and disrupted tight junction protein-maintained barrier function in Caco-2 cells. The pathogenic changes mentioned above were accompanied by an increase in intracellular reactive oxygen species (ROS) levels, lactate dehydrogenase release, and endoplasmic reticulum stress-related signals in the LC-sera or TNFα-pretreated Caco-2 cells. Concomitant incubation of Caco-2 cells with anti-CEACAM1 suppressed these LC-sera or TNFα-induced negative effects on restitution, barrier function, and cell viability.

conclusionThis study demonstrated that sera from cirrhotic patients contain soluble CEACAM1, which is involved in the pathogenesis of intestinal hyperpermeability. Accordingly, it is noteworthy to explore the potential use of anti-CEACAM1 treatment for cirrhosis-related intestinal hyperpermeability and endotoxemia.

Indexed as

PermeabilityApoptosisBiomarkersCell Adhesion Molecule-1Endoplasmic Reticulum StressEpithelial CellsFemaleHumansIntestinesIn Vitro TechniquesLiver CirrhosisMaleMiddle AgedTumor Necrosis Factor-alphaBiomarkersCell Adhesion Molecule-1Tumor Necrosis Factor-alpha

Identifiers

PMID34261981
PMCPMC12966058
OpenAlexW3178295304

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.