Evidence map›Paper›PMID 34261650›Full record

ArticleScience advances2021

The interplay between matrix deformation and the coordination of turning events governs directed neutrophil migration in 3D matrices.

Joshua François, Adithan Kandasamy, Yi-Ting Yeh, Amy Schwartz, Cindy Ayala, Ruedi Meili, Shu Chien, Juan C Lasheras, Juan C Del Álamo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Adaptive PEG Bis-dendron Hydrogels with Tunable Mechanics and Bioactivity.Chemistry of materials : a publication of the American Chemical Society · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Uncertainty-aware traction force microscopy.PLoS computational biology · 2025
    Article
  7. Article
  8. Highway toFrontiers in pharmacology · 2022
    Review
  9. Article
  10. Actin Dynamics as a Multiscale Integrator of Cellular Guidance Cues.Frontiers in cell and developmental biology · 2022
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Joshua FrançoisDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA. juancar@uw.edu joshua_francois@hms.harvard.edu.
Adithan KandasamyDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Yi-Ting YehDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-3002-3425
Amy SchwartzDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Cindy AyalaDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Ruedi MeiliDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Shu ChienDepartment of Bioengineering, University of California, San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-0332-285X
Juan C LasherasDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA.
Juan C Del ÁlamoDepartment of Mechanical and Aerospace Engineering, University of California, San Diego, La Jolla, CA, USA. juancar@uw.edu joshua_francois@hms.harvard.edu.ORCID http://orcid.org/0000-0001-5683-0239

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Integrative Bioengineering of Heart, Vessels, and BloodT32HL105373 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, INTAGLIETTA, MARCOS · 2010 to 2019
$3.6M
Patient-specific thrombosis risk in atrial fibrillation by 4D CT imaging of atrial kinetics combined with computational fluid dynamicsR01HL160024 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEL ALAMO, JUAN CARLOS, KAHN, ANDREW · 2021 to 2024
$2.7M
Quantitative Analysis of Chemotactic Motility Cycle of Ameboid CellsR01GM084227 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DEL ALAMO, JUAN CARLOS, FIRTEL, RICHARD A · 2010 to 2018
$2.7M
NHLBI NIH HHS R01 HL160024NHLBI NIH HHS T32 HL105373NIGMS NIH HHS R01 GM084227NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Neutrophils migrating through extravascular spaces must negotiate narrow matrix pores without losing directional movement. We investigated how chemotaxing neutrophils probe matrices and adjust their migration to collagen concentration ([col]) changes by tracking 20,000 cell trajectories and quantifying cell-generated 3D matrix deformations. In low-[col] matrices, neutrophils exerted large deformations and followed straight trajectories. As [col] increased, matrix deformations decreased, and neutrophils turned often to circumvent rather than remodel matrix pores. Inhibiting protrusive or contractile forces shifted this transition to lower [col], implying that mechanics play a crucial role in defining migratory strategies. To balance frequent turning and directional bias, neutrophils used matrix obstacles as pivoting points to steer toward the chemoattractant. The Actin Related Protein 2/3 complex coordinated successive turns, thus controlling deviations from chemotactic paths. These results offer an improved understanding of the mechanisms and molecular regulators used by neutrophils during chemotaxis in restrictive 3D environments.

Identifiers

PMID34261650
PMCPMC8279509

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.