Evidence map›Paper›PMID 34260926›Full record

ArticleCell reports2021

Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation.

Leilei Xu, Mahboob Ali, Wenxiu Duan, Xiao Yuan, Fatima Garba, McKay Mullen, Binwen Sun, Ina Poser, Hequan Duan, Jianlin Lu and 12 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 7 institutions in 3 countries.

Leilei XuMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Mahboob AliMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.
Wenxiu DuanAnhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.
Xiao YuanMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address: yuanxiao@ustc.edu.cn.
Fatima GarbaKeck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.
McKay MullenMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Binwen SunNational Chromatographic Research and Analysis Center, Dalian 116023, China.
Ina PoserMax Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Hequan DuanMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA; Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.
Jianlin LuMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.
Ruijun TianDepartment of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
Yushu GeAnhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China.
Lingluo ChuMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Weijun PanShanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Dongmei WangMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China.
Anthony HymanMax Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Hadiyah GreenKeck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Lin LiShanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Zhen DouMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Anhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China. Electronic address: douzhen@ustc.edu.cn.
Dan LiuAnhui Key Laboratory for Chemical Biology, Hefei National Center for Physical Sciences at Microscale, Hefei 230027, China. Electronic address: dliu919@ustc.edu.cn.
Xing LiuMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA. Electronic address: xing1017@ustc.edu.cn.
Xuebiao YaoMOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science & Technology of China, Hefei 230027, China; Keck Center for Molecular Imaging, Morehouse School of Medicine, Atlanta, GA 30310, USA. Electronic address: yaoxb@ustc.edu.cn.
University of Science and Technology of China · CNMorehouse School of Medicine · USHefei National Center for Physical Sciences at Nanoscale · CNMax Planck Institute of Molecular Cell Biology and Genetics · DECenter for Excellence in Molecular Cell Science · CNChinese Academy of Sciences · CNSouthern University of Science and Technology · CN

Funding

The function of ezrin in stimulus-coupled acid secretionR01DK056292 · NIDDK · UNIVERSITY OF WISCONSIN MADISON · PI YAO, XUEBIAO · 1999 to 2012
$2.5M
Function of ACAP4 in CCL18-stimulated breast cancer metastasisR01CA164133 · NCI · MOREHOUSE SCHOOL OF MEDICINE · PI YAO, XUEBIAO · 2012 to 2016
$1.4M
FUNCTION OF MST4-EZRIN-ACAP4 SIGNALING IN GASTRIC PARIETAL CELL SECRETION AND HOMEOSTASISR01DK115812 · NIDDK · MOREHOUSE SCHOOL OF MEDICINE · PI YAO, XUEBIAO · 2017 to 2019
$967k
NCI NIH HHS R01 CA164133NIDDK NIH HHS R01 DK056292NIDDK NIH HHS R01 DK115812
6 · The paper itself

Abstract

Stable transmission of genetic material during cell division requires accurate chromosome segregation. PLK1 dynamics at kinetochores control establishment of correct kinetochore-microtubule attachments and subsequent silencing of the spindle checkpoint. However, the regulatory mechanism responsible for PLK1 activity in prometaphase has not yet been affirmatively identified. Here we identify Apolo1, which tunes PLK1 activity for accurate kinetochore-microtubule attachments. Apolo1 localizes to kinetochores during early mitosis, and suppression of Apolo1 results in misaligned chromosomes. Using the fluorescence resonance energy transfer (FRET)-based PLK1 activity reporter, we found that Apolo1 sustains PLK1 kinase activity at kinetochores for accurate attachment during prometaphase. Apolo1 is a cognate substrate of PLK1, and the phosphorylation enables PP1γ to inactivate PLK1 by dephosphorylation. Mechanistically, Apolo1 constitutes a bridge between kinase and phosphatase, which governs PLK1 activity in prometaphase. These findings define a previously uncharacterized feedback loop by which Apolo1 provides fine-tuning for PLK1 to guide chromosome segregation in mitosis.

Indexed as

Chromosome SegregationFeedback, PhysiologicalAmino Acid MotifsAmino Acid SequenceCell Cycle ProteinsHEK293 CellsHeLa CellsHumansKinetochoresMitosisPhosphoprotein PhosphatasesPhosphorylationPhosphoserinePolo-Like Kinase 1Protein BindingProteinsCell Cycle ProteinsPhosphoprotein PhosphatasesPhosphoserinePolo-Like Kinase 1ProteinsProtein Serine-Threonine KinasesProto-Oncogene ProteinsApolo1chromosome segregationkinetochoremitosisPLK1PP1γ

Identifiers

PMID34260926
PMCPMC8358895
OpenAlexW3181310271

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.