ArticleApplied and environmental microbiology2021
Fluorescent Protein Expression as a Proxy for Bacterial Fitness in a High-Throughput Assay.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Efficient Expression of Small Molecule Bioactive Peptides in Bacillus licheniformis.Applied biochemistry and biotechnology · 2026Article
- A versatile dual-color bacterial reporter system highlights two distinctApplied and environmental microbiology · 2026Article
- Chromoprotein-modified plant pathogenic bacteria: tools for experimental tracking and visualization.Microbiology spectrum · 2026Article
- Tracking Inter- and Intraspecific Bacterial Competition in Pairwise Assays Using a Transient Fluorescent Dye.Ecology and evolution · 2026Article
- Enhanced Manganese Oxidation at the Biofilm-Fluid Interface Drives Pore-Scale Patterns in Mineral Precipitation.Environmental science & technology · 2026Article
- Carbon source diversity shapes bacterial interspecies interactions.The ISME journal · 2025Article
- Experimental heatwaves reduce the effectiveness of ejaculates at occupying female reproductive tracts in a model insect.Royal Society open science · 2024Article
- Hitching a Ride in the Phyllosphere: Surfactant Production of Pseudomonas spp. Causes Co-swarming of Pantoea eucalypti 299R.Microbial ecology · 2024Article
- Measuring differential fitness costs and interactions between genetic cassettes using fluorescent spectrophotometry.Applied and environmental microbiology · 2024Article
- Metabolic resource overlap impacts competition among phyllosphere bacteria.The ISME journal · 2023Article
- Exploring Bacterial Attributes That Underpin Symbiont Life in the Monogastric Gut.Applied and environmental microbiology · 2022Review
- Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.PLoS computational biology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial growth is classically assessed by measuring the increases in optical density of pure cultures in shaken liquid media. Measuring growth using optical density has severe limitations when studying multistrain interactions, as it is not possible to measure the growth of individual strains within mixed cultures. Here, we demonstrated that constitutively expressed fluorescent proteins can be used to track the growth of individual strains in different liquid media. Fluorescence measurements were highly correlated with optical density measurements and cell counts. This allowed us to assess bacterial growth not only in pure cultures but also in mixed bacterial cultures and determine the impact of a competitor on a focal strain, thereby assessing relative fitness. Furthermore, we were able to track the growth of two different strains simultaneously by using fluorescent proteins with differential excitation and emission wavelengths. Bacterial densities measured by fluorescence yielded more consistent data between technical replicates than optical density measurements. Our setup employs fluorescence microplate readers that allow high throughput and replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.