Evidence map›Paper›PMID 34260196›Full record

ArticleACS synthetic biology2021

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.

Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn Torger Stokke, Florian Hollfelder, Rahmi Lale

Open access · hybridAbstract read
In one paragraph

Article in ACS synthetic biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Che Fai Alex WongDepartment of Biotechnology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, N-7491, Norway.
Liisa van VlietDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Swapnil Vilas BhujbalDepartment of Biotechnology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, N-7491, Norway.
Chengzhi GuoDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.
Marit SletmoenDepartment of Biotechnology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, N-7491, Norway.
Bjørn Torger StokkeDepartment of Physics, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, N-7491, Norway.ORCID 0000-0003-2991-8088
Florian HollfelderDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, United Kingdom.ORCID 0000-0002-1367-6312
Rahmi LaleDepartment of Biotechnology, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, N-7491, Norway.ORCID 0000-0001-5460-3163
Norwegian University of Science and Technology · NOUniversity of Cambridge · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Water-in-oil emulsion droplets are an attractive format for ultrahigh-throughput screening in functional metagenomics and directed evolution applications that allow libraries with more than 10

Indexed as

Directed Molecular EvolutionGene LibraryHigh-Throughput Screening AssaysMetagenomicsEscherichia colidroplet screeningfunctional metagenomicsmicrofluidicssingle-cell lysissingle-cell screeningsynthetic biology

Identifiers

PMID34260196
PMCPMC8383311
OpenAlexW3180272072

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.