Evidence map›Paper›PMID 34258597›Full record

ArticleSTAR protocols2021

A two-photon FRAP protocol to measure the stereociliary membrane diffusivity in rat cochlear hair cells.

Shefin S George, Charles R Steele, Anthony J Ricci

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Shefin S GeorgeDepartment of Otolaryngology-Head and Neck Surgery, School of Medicine, 240 Pasteur Drive, Stanford University, Stanford, CA 94305, USA.
Charles R SteeleDepartment of Mechanical Engineering, Building 520, 440 Escondido Mall, Stanford University, Stanford, CA 94305, USA.
Anthony J RicciDepartment of Otolaryngology-Head and Neck Surgery, School of Medicine, 240 Pasteur Drive, Stanford University, Stanford, CA 94305, USA.
Stanford University · US

Funding

Tonotopic variations in mechano-electric transductionR01DC003896 · NIDCD · STANFORD UNIVERSITY · PI RICCI, ANTHONY J · 1999 to 2022
$11.1M
Repair of mechano-electrical transduction in mammalian auditory hair cellsR01DC014658 · NIDCD · UNIVERSITY OF KENTUCKY · PI FROLENKOV, GREGORY I · 2015 to 2019
$2.0M
NIDCD NIH HHS R01 DC003896NIDCD NIH HHS R01 DC014658
6 · The paper itself

Abstract

Fluorescence recovery after photobleaching (FRAP) has been widely used to monitor membrane properties by measuring the lateral diffusion of fluorescent particles. This protocol describes how to perform two-photon FRAP on the stereocilia of live cochlear inner hair cells using a lipophilic dye, di-3-ANEPPDHQ, to assess the stereociliary membrane diffusivity. We also detail two-photon FRAP microscope setup and calibration, as well as FRAP parameter setting and data analysis. For complete details on the use and execution of this protocol, please refer to George et al. (2020).

Indexed as

AnimalsColoring AgentsDiffusionFluorescence Recovery After PhotobleachingHair Cells, AuditoryPhotonsRatsStereociliaColoring AgentsCell MembraneMicroscopyMolecular/Chemical ProbesNeuroscience

Identifiers

PMID34258597
PMCPMC8255937
OpenAlexW3175696407

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.