Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides.

Shigeru Tamogami, Ganesh K Agrawal, Randeep Rakwal

Abstract read

In one paragraph

Article in Biochemistry and biophysics reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Single-cell RNA sequencing profiles reveal cell type-specific transcriptional regulation networks conditioning fungal invasion in maize roots.Plant biotechnology journal · 2023
Article

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5 · Who and what money

Authors and funding

3 authors.

Shigeru TamogamiLaboratory of Biologically Active Compounds, Department of Biological Production, Akita Prefectural University, Akita 010-0195, Japan.

Ganesh K AgrawalResearch Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal.

Randeep RakwalResearch Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Development of the methods to examine the molecular targets of biologically active compounds is one of the most important subjects in experimental biology/biochemistry. To evaluate the usability of the (7-nitro-2,1,3-benzoxadiazole)-thioether (NBD-S) probe for this purpose, bioactive chemical probe (1) as the cellulose biosynthesis (CB) inhibitor was synthesized and tested. As a result, a variety of fluorescently-labeled particles and organelles were found in the columella root cap cells of radish plants. Of note, well-defined cellular organelles were clearly recognized in the detaching root cap cells (border-like cells). These results imply that the bioactive NBD-S chemical probe could be a valuable direct-labeling reagent. Analysis of these fluorescent substances would be helpful in providing new information on defined molecular targets and events.

Indexed as

CB, cellulose biosynthesisChemical probeCW, cell wallCys, cysteineDIEA, N,N-diisopropylethylamineDMSO, N,N-dimethylsulfoxideFluorescenceHEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidIndaziflamLys, lysineNBDNBD-Cl, 4-chloro-7-nitro-2,1,3-benzoxadiazoleNBD-N, (7-nitro-2,1,3-benzoxadiazole)-amineNBD, nitrobenzoxadaizoleNBD-O, (7-nitro-2,1,3-benzoxadiazole)-etherNBD-S, (7-nitro-2,1,3-benzoxadiazole)-thioetherRoot capTriaziflam

Identifiers

PMID34258397

DOI10.1016/j.bbrep.2021.101063

PMCPMC8255175

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.