ArticleChemical science2021
Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 17 citations in OpenAlex.
- Photophysics of Organic Fluorophore Photobluing and Its Applications in Fluorescence and Super-Resolution Microscopy.Accounts of chemical research · 2026Article
- Photoactivation of BODIPY Fluorescence with Green Light.The Journal of organic chemistry · 2025Article
- A palette of bridged bicycle-strengthened fluorophores.Nature methods · 2025Article
- Super-photostable organic dye for long-term live-cell single-protein imaging.Nature methods · 2025Article
- Photoactivatable BODIPYs for Live-Cell PALM.Molecules (Basel, Switzerland) · 2023Review
- Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes.Journal of nanobiotechnology · 2022Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.
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Registered trials
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