ArticleNature communications2021
Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- Molecular basis of plant DCL4 action that outcompetes DCL2.Nature plants · 2026Article
- Effective target genes for RNA interference-based management of the cabbage stem flea beetle.Insect molecular biology · 2025Article
- Biochemical and structural basis of Dicer helicase function unveiled by resurrecting ancient proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- In vivo Dicer-2 interactome during viral infection reveals novel pro and antiviral factors in Drosophila melanogaster.PLoS pathogens · 2025Article
- DsRNA as pathogen-associated molecular pattern in innate immunity and multiple functions of the RNAi machinery complicate the use of RNAi in pest control.Frontiers in insect science · 2025Review
- Article
- Size-Selective Capturing of Exosomes Using DNA Tripods.Journal of the American Chemical Society · 2024Article
- The Complex Landscape of Structural Divergence Between the Drosophila pseudoobscura and D. persimilis Genomes.Genome biology and evolution · 2024Article
- Review
- microRNAs in action: biogenesis, function and regulation.Nature reviews. Genetics · 2023Review
- Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.Nature communications · 2023Article
- Dicer structure and function: conserved and evolving features.EMBO reports · 2023Review
- Ancestral protein reconstruction reveals evolutionary events governing variation in Dicer helicase function.eLife · 2023Article
- Revised Annotation and Characterization of NovelBiology · 2022Article
- Article
- Article
- Functional specialization of monocot DCL3 and DCL5 proteins through the evolution of the PAZ domain.Nucleic acids research · 2022Article
- Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila Dicer-2 (Dcr-2) produces small interfering RNAs from long double-stranded RNAs (dsRNAs), playing an essential role in antiviral RNA interference. The dicing reaction by Dcr-2 is enhanced by Loquacious-PD (Loqs-PD), a dsRNA-binding protein that partners with Dcr-2. Previous biochemical analyses have proposed that Dcr-2 uses two distinct-processive or distributive-modes of cleavage by distinguishing the terminal structures of dsRNAs and that Loqs-PD alters the terminal dependence of Dcr-2. However, the direct evidence for this model is lacking, as the dynamic movement of Dcr-2 along dsRNAs has not been traced. Here, by utilizing single-molecule imaging, we show that the terminal structures of long dsRNAs and the presence or absence of Loqs-PD do not essentially change Dcr-2's cleavage mode between processive and distributive, but rather simply affect the probability for Dcr-2 to undergo the cleavage reaction. Our results provide a refined model for how the dicing reaction by Dcr-2 is regulated.
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