Evidence map›Paper›PMID 34256244›Full record

ArticleVirology2021

Isolation of a novel insect-specific flavivirus with immunomodulatory effects in vertebrate systems.

Albert J Auguste, Rose M Langsjoen, Danielle L Porier, Jesse H Erasmus, Nicholas A Bergren, Bethany G Bolling, Huanle Luo, Ankita Singh, Hilda Guzman, Vsevolod L Popov and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
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  5. Differential effect of acuteOne health (Amsterdam, Netherlands) · 2025
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  15. Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.Proceedings of the National Academy of Sciences of the United States of America · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 2 countries.

Albert J AugusteDepartment of Entomology, College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA; Center for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA. Electronic address: jauguste@vt.edu.
Rose M LangsjoenDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Danielle L PorierDepartment of Entomology, College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA.
Jesse H ErasmusDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Nicholas A BergrenDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Bethany G BollingDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Huanle LuoDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Ankita SinghDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Hilda GuzmanDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Vsevolod L PopovDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Amelia P A Travassos da RosaDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Tian WangDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Lin KangEdward Via College of Osteopathic Medicine, Monroe, LA, 71203, USA; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, 24060, USA.
Irving C AllenCenter for Emerging, Zoonotic, and Arthropod-borne Pathogens, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, 24060, USA.
Christine V F CarringtonDepartment of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Robert B TeshDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Scott C WeaverDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
The University of Texas Medical Branch at Galveston · USVirginia–Maryland College of Veterinary Medicine · USVirginia Tech · USUniversity of the West Indies · TT

Funding

World Reference Center for Emerging Viruses and Arboviruses (WRCEVA)R24AI120942 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI WEAVER, SCOTT C · 2016 to 2024
$8.5M
Trajectory of Vector-adaptive Chikungunya Virus EvolutionR01AI121452 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI WEAVER, SCOTT C · 2016 to 2020
$2.3M
A Novel Strategy for Generating Safe and Effective Flavivirus VaccinesR01AI153433 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI AUGUSTE, ALBERT J. · 2020 to 2024
$2.0M
Mechanisms by which insect specific flaviviruses are host restricted and preclude super-infection with pathogenic flavivirusesK22AI125474 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI AUGUSTE, ALBERT J. · 2018 to 2019
$266k
NIAID NIH HHS K22 AI125474NIAID NIH HHS R01 AI121452NIAID NIH HHS R01 AI153433NIAID NIH HHS R24 AI120942
6 · The paper itself

Abstract

We describe the isolation and characterization of a novel insect-specific flavivirus (ISFV), tentatively named Aripo virus (ARPV), that was isolated from Psorophora albipes mosquitoes collected in Trinidad. The ARPV genome was determined and phylogenetic analyses showed that it is a dual host associated ISFV, and clusters with the main mosquito-borne flaviviruses. ARPV antigen was significantly cross-reactive with Japanese encephalitis virus serogroup antisera, with significant cross-reactivity to Ilheus and West Nile virus (WNV). Results suggest that ARPV replication is limited to mosquitoes, as it did not replicate in the sandfly, culicoides or vertebrate cell lines tested. We also demonstrated that ARPV is endocytosed into vertebrate cells and is highly immunomodulatory, producing a robust innate immune response despite its inability to replicate in vertebrate systems. We show that prior infection or coinfection with ARPV limits WNV-induced disease in mouse models, likely the result of a robust ARPV-induced type I interferon response.

Indexed as

ImmunomodulationAnimalsAntigens, ViralCross ReactionsCulicidaeDisease Models, AnimalFlavivirusGenome, ViralHost SpecificityImmunity, InnateInsect VirusesMacrophagesMicePhylogenyVertebratesViral InterferenceAntigens, ViralAripo virusFlavivirus infectionFlavivirus pathogenesisInsect-specific flavivirusSuper-infection exclusionWest nile virus

Identifiers

PMID34256244
PMCPMC8419152
OpenAlexW3178567784

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.