ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2021
Chromosome-scale genome assembly of the transformation-amenable common wheat cultivar 'Fielder'.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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89 citing papers in PubMed, 148 citations in OpenAlex.
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- Genomic and genetic dissection of drought tolerance in a resilient wheat germplasm JIN50.Nature genetics · 2026Article
- Wheat's war against stripe rust: Integrating host immunity, genomics and breeding for durable resistance.The plant genome · 2026Review
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Genome-wide characterization and adaptive evolution of favorable gibberellin 2-oxidase alleles contributing to wheat agronomic traits.Journal of advanced research · 2026Article
- A high-quality bread wheat genome unravels the adaptive evolution of wheat end-use quality.Nature plants · 2026Article
- TaERF109: A Novel ERF Transcription Factor Contributing to Enhanced Resistance toPathogens (Basel, Switzerland) · 2026Article
- Review
- Current status and future perspective of genomics-assisted breeding in wheat (Breeding science · 2026Article
- Genomics in wheat improvement: Progress and perspectives.The plant genome · 2026Review
- Large-scale plant genomic identification and analysis uncoverHorticulture research · 2026Article
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Whole genome identification and expression analysis ofFrontiers in plant science · 2026Article
- Role of chromosome ends in meiotic stability, recombination and wheat evolution in the context of breeding.BMC plant biology · 2025Article
- BS-D275: A novel locus on the chromosome arm 2DS governing branched spike development in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Paired NLRs originated from Triticum dicoccoides coordinately confer resistance to powdery mildew in wheat.Nature communications · 2025Article
- The impact of asymmetrical gene expression on the development of spike morphology iniMetaOmics · 2025Article
- Comprehensive analysis of bHLH genes in wheat and functional characterization of TabHLH319 in salt tolerance.Plant cell reports · 2025Article
- Reference-based chromosome-scale assembly of Japanese barley (Hordeum vulgare ssp. vulgare) cultivar Hayakiso 2.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
We have established a high-quality, chromosome-level genome assembly for the hexaploid common wheat cultivar 'Fielder', an American, soft, white, pastry-type wheat released in 1974 and known for its amenability to Agrobacterium tumefaciens-mediated transformation and genome editing. Accurate, long-read sequences were obtained using PacBio circular consensus sequencing with the HiFi approach. Sequence reads from 16 SMRT cells assembled using the hifiasm assembler produced assemblies with N50 greater than 20 Mb. We used the Omni-C chromosome conformation capture technique to order contigs into chromosome-level assemblies, resulting in 21 pseudomolecules with a cumulative size of 14.7 and 0.3 Gb of unanchored contigs. Mapping of published short reads from a transgenic wheat plant with an edited seed-dormancy gene, TaQsd1, identified four positions of transgene insertion into wheat chromosomes. Detection of guide RNA sequences in pseudomolecules provided candidates for off-target mutation induction. These results demonstrate the efficiency of chromosome-scale assembly using PacBio HiFi reads and their application in wheat genome-editing studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.