Evidence map›Paper›PMID 34251541›Full record

ArticleCell and tissue banking2022

Improved cell seeding efficiency and cell distribution in porous hydroxyapatite scaffolds by semi-dynamic method.

Feng Shi, Ke Duan, Zaijun Yang, Yumei Liu, Jie Weng

Abstract read
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In one paragraph

Article in Cell and tissue banking, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Feng ShiCollaboration and Innovation Center of Tissue Repair Material Engineering Technology, China West Normal University, Nanchong, 637009, Sichuan, China.ORCID http://orcid.org/0000-0003-1337-5293
Ke DuanSichuan Provincial Laboratory of Orthopaedic Engineering, Department of Orthopaedics, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.
Zaijun YangCollaboration and Innovation Center of Tissue Repair Material Engineering Technology, China West Normal University, Nanchong, 637009, Sichuan, China.
Yumei LiuCollaboration and Innovation Center of Tissue Repair Material Engineering Technology, China West Normal University, Nanchong, 637009, Sichuan, China. liuyumei@cwnu.edu.cn.
Jie WengChina Key Laboratory of Advanced Technologies of Materials, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China. jweng@swjtu.edu.cn.
China West Normal University · CNAffiliated Hospital of Southwest Medical University · CNSouthwest Jiaotong University · CN

Funding

Innovative Research Group Project of the National Natural Science Foundation of China 2016YFB0700803Luzhou-SWMU Strategic Cooperation Program 2017LZXNYD-J35Major Project of Education Department in Sichuan Province 18CZ0019Science & Technology Department of Sichuan Province 2020065, 21MZGC0218Startup Program of Southwest Normal University 18Q069, 18Q030
6 · The paper itself

Abstract

Tissue engineering is a promising technique for the repair of bone defects. An efficient and homogeneous distribution of cell seeding into scaffold is a crucial but challenging step in the technique. Murine bone marrow mesenchymal stem cells were seeded into porous hydroxyapatite scaffolds of two morphologies by three methods: static seeding, semi-dynamic seeding, or dynamic perfusion seeding. Seeding efficiency, survival, distribution, and proliferation were quantitatively evaluated. To investigate the performance of the three seeding methods for larger/thicker scaffolds as well as batch seeding of numerous scaffolds, three scaffolds were stacked to form assemblies, and seeding efficiencies and cell distribution were analyzed. The semi-dynamic seeding and static seeding methods produced significantly higher seeding efficiencies, vitalities, and proliferation than did the dynamic perfusion seeding. On the other hand, the semi-dynamic seeding and dynamic perfusion seeding methods resulted in more homogeneous cell distribution than did the static seeding. For stacked scaffold assemblies, the semi-dynamic seeding method also created superior seeding efficiency and longitudinal cell distribution homogeneity. The semi-dynamic seeding method combines the high seeding efficiency of static seeding and satisfactory distribution homogeneity of dynamic seeding while circumventing their disadvantages. It may contribute to improved outcomes of bone tissue engineering.

Indexed as

DurapatiteMesenchymal Stem CellsAnimalsBone and BonesCells, CulturedMicePorosityTissue EngineeringTissue ScaffoldsDurapatiteBatch samplesCell seedingPorous scaffoldSemi-dynamic seeding

Identifiers

PMID34251541
OpenAlexW3182629306

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.