Evidence map›Paper›PMID 34251336›Full record

ArticleeLife2021

Structural intermediates observed only in intact

Thushani D Nilaweera, David A Nyenhuis, David S Cafiso

Open access · goldAbstract read
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
  2. Review
  3. Assimilation of phthalate esters in bacteria.Applied microbiology and biotechnology · 2024
    Review
  4. PMGut microbes · 2023
    Article
  5. Article
  6. Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex inAngewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2023
    Article
  7. Article
  8. Article
  9. GdJournal of the American Chemical Society · 2023
    Article
  10. Article
  11. HDX-MS performed on BtuB inProceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Thushani D Nilaweera *Department of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, United States.
David A Nyenhuis *Department of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, United States.
David S CafisoDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, United States.ORCID 0000-0002-3813-8721
University of Virginia · US

Funding

MOLECULAR MECHANISMS OF MEMBRANE TRANSPORTR01GM035215 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI DAVID S CAFISO · 1985 to 2026
$7.3M
Training in Molecular BiophysicsT32GM080186 · NIGMS · UNIVERSITY OF VIRGINIA · PI NAKAMOTO, ROBERT K. · 2008 to 2019
$1.5M
Upgrade of Bruker E500 EPR spectrometerS10RR027152 · NCRR · UNIVERSITY OF VIRGINIA · PI CAFISO, DAVID S · 2010 to 2010
$500k
Upgrade of Bruker EPR SpectrometerS10OD025149 · OD · UNIVERSITY OF VIRGINIA · PI CAFISO, DAVID S · 2018 to 2018
$396k
NCRR NIH HHS S10 RR027152NIGMS NIH HHS R01 GM035215NIGMS NIH HHS T32 GM080186NIH HHS S10 OD025149
6 · The paper itself

Abstract

Outer membrane TonB-dependent transporters facilitate the uptake of trace nutrients and carbohydrates in Gram-negative bacteria and are essential for pathogenic bacteria and the health of the microbiome. Despite this, their mechanism of transport is still unknown. Here, pulse electron paramagnetic resonance (EPR) measurements were made in intact cells on the

Indexed as

Bacterial Outer Membrane ProteinsBiological TransportElectron Spin Resonance SpectroscopyEscherichia coliEscherichia coli ProteinsHumansMembrane ProteinsMembrane Transport ProteinsModels, MolecularPeriplasmProtein BindingProtein ConformationVitamin B 12Bacterial Outer Membrane ProteinsBtuB protein, E coliEscherichia coli ProteinsMembrane ProteinsMembrane Transport ProteinstonB protein, E coliVitamin B 12double electron-electron resonanceE. coliinfectious diseasein-situ EPR spectroscopymicrobiologymolecular biophysicssite-directed spin labelingstructural biologyTonB-dependent transport

Identifiers

PMID34251336
PMCPMC8341980
OpenAlexW3178621785

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.