ArticleeLife2021
The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The trial behind it
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- Monovalent cations differentially regulate the activity of eukaryotic fluoride channels and impact fluoride resistance.PLoS biology · 2026Article
- Asymmetry-induced distinct mechanisms and the transporting role of sodium in bacterial fluoride channel Fluc.bioRxiv : the preprint server for biology · 2026Article
- The molecular mechanism of fluoride export by the eukaryotic fluoride channel FEX.Nature communications · 2025Article
- Facts and Fallacies of the Fluoride Controversy: A Contemporary Perspective.International dental journal · 2025Review
- Molecular Insights into Fluoride Ion Uptake and Selectivity in the CLCF Fluoride/Proton Antiporter.The journal of physical chemistry. B · 2025Article
- Fluorinated Protein-Ligand Complexes: A Computational Perspective.The journal of physical chemistry. B · 2024Review
- The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis.Nature communications · 2024Review
- Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances.Applied and environmental microbiology · 2024Review
- Transport of metformin metabolites by guanidinium exporters of the small multidrug resistance family.The Journal of general physiology · 2024Article
- RecombinantmBio · 2024Article
- Lanthanum-fluoride electrode-based methods to monitor fluoride transport in cells and reconstituted lipid vesicles.Methods in enzymology · 2024Article
- Measurement and analysis of microbial fluoride resistance in dental biofilm models.Methods in enzymology · 2024Article
- Dimerization mechanism of an inverted-topology ion channel in membranes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism inEnvironmental science & technology · 2023Article
- Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR.Science advances · 2023Article
- Ion permeation, selectivity, and electronic polarization in fluoride channels.Biophysical journal · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fluc family fluoride channels protect microbes against ambient environmental fluoride by undermining the cytoplasmic accumulation of this toxic halide. These proteins are structurally idiosyncratic, and thus the permeation pathway and mechanism have no analogy in other known ion channels. Although fluoride-binding sites were identified in previous structural studies, it was not evident how these ions access aqueous solution, and the molecular determinants of anion recognition and selectivity have not been elucidated. Using x-ray crystallography, planar bilayer electrophysiology, and liposome-based assays, we identified additional binding sites along the permeation pathway. We used this information to develop an oriented system for planar lipid bilayer electrophysiology and observed anion block at one of these sites, revealing insights into the mechanism of anion recognition. We propose a permeation mechanism involving alternating occupancy of anion-binding sites that are fully assembled only as the substrate approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.