ArticleCell proliferation2021
SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre-implantation embryo development.
Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- A systematic single-cell analysis of enhancer RNAs across human and mouse.Nucleic acids research · 2026Article
- Enhancing gene expression studies in bovine embryos fertilized in vitro: Identifying stable reference genes across blastocysts with different developmental speeds.The Journal of reproduction and development · 2025Article
- Microtubule Integrity Is Associated with Mitochondrial Function and Quality of Murine Preimplantation Embryos.International journal of molecular sciences · 2025Article
- Oleanolic acid improves the in vitro developmental competence of early porcine embryos by reducing oxidative stress and ameliorating mitochondrial function.Animal bioscience · 2025Article
- NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.Animal bioscience · 2023Article
- Overnight Corticosterone and Gene Expression in Mouse Hippocampus: Time Course during Resting Period.International journal of molecular sciences · 2023Article
- Neurotrophin-4 promotes the specification of trophectoderm lineage after parthenogenetic activation and enhances porcine early embryonic development.Frontiers in cell and developmental biology · 2023Article
- Improvement of Fertilization Capacity and Developmental Ability of Vitrified Bovine Oocytes by JUNO mRNA Microinjection and Cholesterol-Loaded Methyl-β-Cyclodextrin Treatment.International journal of molecular sciences · 2022Article
- Changes in OCT4 expression play a crucial role in the lineage specification and proliferation of preimplantation porcine blastocysts.Cell proliferation · 2022Article
- Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The PorcineStem cells international · 2022Article
- Molecular Mechanisms Underlying the Inhibition of Proliferation and Differentiation by Florfenicol in P19 Stem Cells: Transcriptome Analysis.Frontiers in pharmacology · 2022Article
- SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre-implantation embryo development.Cell proliferation · 2021Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
objectivesGene regulation in early embryos has been widely studied for a long time because lineage segregation gives rise to the formation of a pluripotent cell population, known as the inner cell mass (ICM), during pre-implantation embryo development. The extraordinarily longer pre-implantation embryo development in pigs leads to the distinct features of the pluripotency network compared with mice and humans. For these reasons, a comparative study using pre-implantation pig embryos would provide new insights into the mammalian pluripotency network and help to understand differences in the roles and networks of genes in pre-implantation embryos between species. MATERIALS AND
methodsTo analyse the functions of SOX2 in lineage segregation and cell proliferation, loss- and gain-of-function studies were conducted in pig embryos using an overexpression vector and the CRISPR/Cas9 system. Then, we analysed the morphological features and examined the effect on the expression of downstream genes through immunocytochemistry and quantitative real-time PCR.
resultsOur results showed that among the core pluripotent factors, only SOX2 was specifically expressed in the ICM. In SOX2-disrupted blastocysts, the expression of the ICM-related genes, but not OCT4, was suppressed, and the total cell number was also decreased. Likewise, according to real-time PCR analysis, pluripotency-related genes, excluding OCT4, and proliferation-related genes were decreased in SOX2-targeted blastocysts. In SOX2-overexpressing embryos, the total blastocyst cell number was greatly increased but the ICM/TE ratio decreased.
conclusionsTaken together, our results demonstrated that SOX2 is essential for ICM formation and cell proliferation in porcine early-stage embryogenesis.
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