ArticleBio-protocol2021
Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions.
Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Tubulin autoregulation mediator TTC5 regulates neuronal morphology and migration.bioRxiv : the preprint server for biology · 2026Article
- Robust mammalian RNA localization elements are complex and multipartite.bioRxiv : the preprint server for biology · 2026Article
- Locally synthesized glycyl aminoacyl-tRNA synthetase is important for local translation in neurons.Life science alliance · 2026Article
- Article
- Circular RNAScience advances · 2026Article
- TDP-43 directly inhibits mRNA accumulation in neurites through modulation of mRNA stability.The EMBO journal · 2026Article
- The RNA-binding protein HNRNPA2B1 regulates neurite RNA abundance and motor-dependent cargo transport.Molecular biology of the cell · 2025Article
- Analyzing RNA Localization Using the RNA Proximity Labeling Method OINC-seq.Bio-protocol · 2025Article
- Quantification of subcellular RNA localization through direct detection of RNA oxidation.Nucleic acids research · 2025Article
- Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis.Nature communications · 2024Article
- Quantification of subcellular RNA localization through direct detection of RNA oxidation.bioRxiv : the preprint server for biology · 2024Article
- Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis.Research square · 2023Article
- Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes.Nature communications · 2023Article
- Article
- High-throughput identification of RNA localization elements in neuronal cells.Nucleic acids research · 2022Article
- Halo-seq: An RNA Proximity Labeling Method for the Isolation and Analysis of Subcellular RNA Populations.Current protocols · 2022Article
- Transcriptome-scale methods for uncovering subcellular RNA localization mechanisms.Biochimica et biophysica acta. Molecular cell research · 2022Review
- The Role of Alternative Polyadenylation in the Regulation of Subcellular RNA Localization.Frontiers in genetics · 2021Review
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Authors and funding
4 authors.
Funding
Abstract
Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the characterization of the transcripts present at a given subcellular location. Historically, experiments aimed at answering this question have centered upon microscopy-based techniques that target one or a few transcripts at a time. However, more recently, the advent of high-throughput RNA sequencing has allowed the transcriptome-wide profiling of the RNA content of subcellular fractions. Here, we present a protocol for the isolation of cell body and neurite fractions from neuronal cells using mechanical fractionation and characterization of their RNA content. Graphic abstract: Fractionation of neuronal cells and analysis of subcellular RNA contents.
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