ArticleFrontiers in veterinary science2021
Design of a High-Throughput Real-Time PCR System for Detection of Bovine Respiratory and Enteric Pathogens.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Molecular epidemiology, seroprevalence and genetic characterization of bovine rotavirus in Qinghai yaks: first identification of G6P[5] genotype.Archives of virology · 2026Article
- Occurrence of respiratory and enteric bacteria and viruses in calves in Iceland.Acta veterinaria Scandinavica · 2026Article
- Bovine Respiratory Mycoplasmas and the Commensal-Pathogen Continuum: A Systematic Review of Vaccines and Diagnostic Approaches.Animals : an open access journal from MDPI · 2026Review
- Influenza A virus infection dynamics in two sow herds and effects of interventions.Porcine health management · 2026Article
- First Isolation of Bovine Coronavirus From Yanbian, China, and Analytical Validation of a SYBR Green I RT-qPCR Panel for Calf Diarrhea Viruses.Transboundary and emerging diseases · 2026Article
- Quantification of major bacterial pathogens in liver abscesses and matched ruminal and colonic epithelial tissues of feedlot cattle.Microbiology spectrum · 2025Article
- Pathogen-driven pregnancy loss in dairy cattle: An overview of diagnostic advances and innovations.Open veterinary journal · 2025Review
- Establishment and epidemiological investigation of a dual fluorescent qPCR assay forFrontiers in cellular and infection microbiology · 2025Article
- Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus.Veterinary sciences · 2024Review
- Frequency of Bovine Respiratory Disease Complex Bacterial and Viral Agents Using Multiplex Real-Time qPCR in Quebec, Canada, from 2019 to 2023.Veterinary sciences · 2024Article
- Exploring the Genetic Diversity ofMicroorganisms · 2024Article
- Bovine Respiratory Disease (BRD) in Post-Weaning Calves with Different Prevention Strategies and the Impact on Performance and Health Status.Animals : an open access journal from MDPI · 2024Article
- Comparing Occurrence of Bovine Respiratory Pathogens Detected by High-Throughput Real-Time PCR in Nasal Swabs and Non-Endoscopic Bronchoalveolar Lavage Samples from Dairy and Veal Calves.Pathogens (Basel, Switzerland) · 2024Article
- Monitoring bovine dairy calf health and related risk factors in the first three months of rearing.Acta veterinaria Scandinavica · 2023Article
- Gastro-intestinal lesions are not relatable to diarrhoea or specific pathogens in post-weaning diarrhoea (PWD) in pigs.Acta veterinaria Scandinavica · 2023Article
- Lesions and pathogens found in pigs that died during the nursery period in five Danish farms.Porcine health management · 2023Article
- Article
- Characterization of Influenza D Virus in Danish Calves.Viruses · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine respiratory and enteric diseases have a profound negative impact on animal, health, welfare, and productivity. A vast number of viruses and bacteria are associated with the diseases. Pathogen detection using real-time PCR (rtPCR) assays performed on traditional rtPCR platforms are costly and time consuming and by that limit the use of diagnostics in bovine medicine. To diminish these limitations, we have developed a high-throughput rtPCR system (BioMark HD; Fluidigm) for simultaneous detection of the 11 most important respiratory and enteric viral and bacterial pathogens. The sensitivity and specificity of the rtPCR assays on the high-throughput platform was comparable with that of the traditional rtPCR platform. Pools consisting of positive and negative individual field samples were tested in the high-throughput rtPCR system in order to investigate the effect of an individual sample in a pool. The pool tests showed that irrespective of the size of the pool, a high-range positive individual sample had a high influence on the cycle quantification value of the pool compared with the influence of a low-range positive individual sample. To validate the test on field samples, 2,393 nasal swab and 2,379 fecal samples were tested on the high-throughput rtPCR system as pools in order to determine the occurrence of the 11 pathogens in 100 Danish herds (83 dairy and 17 veal herds). In the dairy calves,
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