Evidence map›Paper›PMID 34248070›Full record

ArticleThe Journal of reproduction and development2021

Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis).

Shinichi Watanabe, Megumi Miura, Hiromi Morita, Moeka Nishi, Shin-Ichi Yokota, Shosaku Hattori, Hiromichi Matsumoto, Emiko Fukui, Ken Takeshi Kusakabe, Masanori Ochi and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of reproduction and development, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Shinichi WatanabeOchi Yume Clinic Nagoya, Aichi 460-0002, Japan.
Megumi MiuraOchi Yume Clinic Nagoya, Aichi 460-0002, Japan.
Hiromi MoritaOchi Yume Clinic Nagoya, Aichi 460-0002, Japan.
Moeka NishiGraduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.
Shin-Ichi YokotaAmami Laboratory, Institute of Medical Science, The University of Tokyo, Kagoshima 894-1531, Japan.
Shosaku HattoriAmami Laboratory, Institute of Medical Science, The University of Tokyo, Kagoshima 894-1531, Japan.
Hiromichi MatsumotoGraduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.
Emiko FukuiGraduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.
Ken Takeshi KusakabeJoint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
Masanori OchiOchi Yume Clinic Nagoya, Aichi 460-0002, Japan.
Naomi NakagataDivision of Reproductive Biotechnology and Innovation, Centre for Animal Resources and Development, Kumamoto University, Kumamoto 860-0811, Japan.
Yasuo KisoJoint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan.
Chieko KaiAmami Laboratory, Institute of Medical Science, The University of Tokyo, Kagoshima 894-1531, Japan.
Midori YoshizawaGraduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.
Utsunomiya University · JPThe University of Tokyo · JPYamaguchi University · JPKumamoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. The unilateral ovary was excised after the administration of human chorionic gonadotropin (hCG), to induce ovulation, following which the larger developed follicular oocytes were collected. Follicular oocytes were prepared for ICSI using sperm from the epididymal tail of a unilateral testis extracted from a mature male. The embryos were continuously incubated in CMRL 1066 medium supplemented with 10% (v/v) fetal bovine serum. Embryo culture was performed with cumulus cells. Two experiments of ICSI carried out with three females resulted in 14 mature oocytes from the 49 cumulus-oocyte complexes collected and five embryos, three of which developed into blastocysts. These blastocysts were vitrified, thawed, and transferred to recipient monkeys, but no pregnancies resulted. In conclusion, the present study is the first to successfully produce ICSI-derived blastocysts from MII oocytes obtained by means of hormone administration (a combination of eCG+inhibin antiserum and hCG) and in vitro maturation in immature squirrel monkeys.

Indexed as

AnimalsBlastocystCryopreservationEmbryo Culture TechniquesEmbryo TransferEndangered SpeciesFemaleIn Vitro Oocyte Maturation TechniquesMaleOocyte RetrievalPregnancyPregnancy OutcomeSaimiriSperm Injections, IntracytoplasmicBlastocystIntracytoplasmic sperm injectionsIn vitro maturation of oocytesSquirrel monkey (Saimiri boliviensis)Superovulation

Identifiers

PMID34248070
PMCPMC8423609
OpenAlexW3178604756

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.