ArticlePlant methods2021
A cassava common mosaic virus vector for virus-induced gene silencing in cassava.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review.Current microbiology · 2026Review
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Identification of efficient geminivirus-derived LIR-elements for exogenous protein expression.Archives of virology · 2026Article
- Phytomelatonin receptor PMTR1 is crucial for melatonin-mediated plant broad-spectrum disease resistance.Plant cell reports · 2026Article
- Plant Viral Vectors for Vaccine Development.Vaccines · 2026Review
- Viral vectors for antimicrobial peptide expression: a new path for crop protection.Frontiers in microbiology · 2026Review
- miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.Molecular plant pathology · 2025Article
- Hairpin inserts in viral genomes are stable when they conform to the thermodynamic properties of viral RNA substructures.Journal of virology · 2025Article
- Systematic Analysis of Cinnamyl Alcohol Dehydrogenase Family in Cassava and Validation ofInternational journal of molecular sciences · 2024Article
- Genome-wide analysis of the common bean (Phaseolus vulgaris) laccase gene family and its functions in response to abiotic stress.BMC plant biology · 2024Article
- Metabolic GWAS-based dissection of genetic basis underlying nutrient quality variation and domestication of cassava storage root.Genome biology · 2023Article
- Superoxide dismutase gene family in cassava revealed their involvement in environmental stress via genome-wide analysis.iScience · 2023Article
- MeGLYI-13, a Glyoxalase I Gene in Cassava, Enhances the Tolerance of Yeast andPlants (Basel, Switzerland) · 2023Article
- Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava.Plant methods · 2023Article
- Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.Molecular plant pathology · 2023Review
- Magnesium chelatase subunit D is not only required for chlorophyll biosynthesis and photosynthesis, but also affecting starch accumulation in Manihot esculenta Crantz.BMC plant biology · 2023Article
- A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants.Plant communications · 2023Article
- Genome-wide identification and characterization ofFrontiers in plant science · 2023Article
- Integrative analysis of metabolome and transcriptome reveals the mechanism of color formation in cassava (Frontiers in plant science · 2023Article
- Cassava Frogskin Disease: Current Knowledge on a Re-Emerging Disease in the Americas.Plants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing.
resultsIn this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months.
conclusionsThis CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.
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