ArticleBiotechnology and applied biochemistry2022
Determination of human COVID-19 total antibodies in serum using a time-resolved fluorescence immunoassay.
Article in Biotechnology and applied biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- A Novel Time-resolved Fluorescence Immunochromatographic Strip for the Diagnosis of Avian Infectious Bronchitis Virus.Journal of fluorescence · 2025Article
- Advances in Biosensing Technologies for Diagnosis of COVID-19.Biosensors · 2022Review
- Determination of human COVID-19 total antibodies in serum using a time-resolved fluorescence immunoassay.Biotechnology and applied biochemistry · 2022Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spreading rapidly around the world. Antibody detection plays an important role in the diagnosis of COVID-19. Here, we established a new time-resolved fluorescence immunoassay (TRFIA) to determine COVID-19 total antibodies. A double-antigen sandwich TRFIA was optimized and established: recombinant nucleocapsid phosphoprotein (N protein) and spike protein (S protein) of COVID-19 immobilized on 96-well plates captured human COVID-19 antibodies and then banded together with the N/S proteins labeled with europium(III) (Eu
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