ArticleVeterinary medicine and science2021
Genotyping of Brucella strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA-15.
Article in Veterinary medicine and science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses.Veterinary research communications · 2024Review
- New insights into the genetic predisposition of brucellosis and its effect on the gut and vaginal microbiota in goats.Scientific reports · 2023Article
- Genotyping of Brucella strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA-15.Veterinary medicine and science · 2021Article
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8 authors.
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Abstract
backgroundPrevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples. Designed primers of B.abortus for cattle and B.melitensis for humans were utilised to perform PCR. Culturing and isolation was carried to further to perform MLVA genotyping assay through the selection of two panels of primer markers.
resultsRBPT showed more number of cases of brucellosis in animals and humans compared to the PCR findings. Genotyping findings based upon MLVA-15 set of markers demonstrated that the isolated strains of B.abortus fall in the same clade with strain A1, P8 and A2 from Pakistan and also similar with BCCN#02-45 strain from India. On the other hand, B.melitensis isolated from different districts of Pakistan shared the same clade with BwIM-AFG 63, BwIM-AFG 44 strains from Afghanistan and BwIM IRN 37 strain from Iran. Selected VNTR alleles were sequenced for calibration purposes.
conclusionIt is concluded that Brucella is prevalent in animals and humans in studied districts of Pakistan. Moreover, A1, P8, BwIM-AFG 63, BwIM-AFG 44 and A2 were found the common genotypes in Pakistan.
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