Evidence map›Paper›PMID 34244893›Full record

ArticlePharmaceutical research2021

Multi-Electrode Array of Sensory Neurons as an In Vitro Platform to Identify the Nociceptive Response to Pharmaceutical Buffer Systems of Injectable Biologics.

Muriel Eaton, Zhefu Que, Jingliang Zhang, Kaethe Beck, Riyi Shi, Jeff McDermott, Michael Ladisch, Yang Yang

Abstract read
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In one paragraph

Article in Pharmaceutical research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Muriel EatonDepartment of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Hall for Discovery and Learning Research (DLR), 207 S Martin Jischke Dr., West Lafayette, Indiana, USA.
Zhefu QueDepartment of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Hall for Discovery and Learning Research (DLR), 207 S Martin Jischke Dr., West Lafayette, Indiana, USA.
Jingliang ZhangDepartment of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Hall for Discovery and Learning Research (DLR), 207 S Martin Jischke Dr., West Lafayette, Indiana, USA.
Kaethe BeckOffice of the Executive Vice President for Research and Partnerships, Purdue University, West Lafayette, Indiana, USA.
Riyi ShiPurdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, Indiana, USA.
Jeff McDermottEli Lilly and Company, Delivery Devices and Connected Solutions, Indianapolis, Indiana, USA.
Michael LadischDepartment of Agricultural and Biological Engineering, College of Engineering; Weldon School of Biomedical Engineering; and Laboratory of Renewable Resources Engineering (LORRE), Purdue University, 225 S. University Street, West Lafayette, Indiana, USA. ladisch@purdue.edu.
Yang YangDepartment of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Hall for Discovery and Learning Research (DLR), 207 S Martin Jischke Dr., West Lafayette, Indiana, USA. yangyang@purdue.edu.ORCID http://orcid.org/0000-0001-6417-3654
Purdue University West Lafayette · USEli Lilly (United States) · US

Funding

National Science Foundation: Graduate Research Fellowship DGE-1842166
6 · The paper itself

Abstract

purposePharmaceutical buffer systems, especially for injectable biologics such as monoclonal antibodies, are an important component of successful FDA-approved medications. Clinical studies indicate that buffer components may be contributing factors for increased injection site pain.

methodsTo determine the potential nociceptive effects of clinically relevant buffer systems, we developed an in vitro multi-electrode array (MEA) based recording system of rodent dorsal root ganglia (DRG) sensory neuron cell culture. This system monitors sensory neuron activity/firing as a surrogate of nociception when challenged with buffer components used in formulating monoclonal antibodies and other injectable biologics.

resultsWe show that citrate salt and citrate mannitol buffer systems cause an increase in mean firing rate, burst frequency, and burst duration in DRG sensory neurons, unlike histidine or saline buffer systems at the same pH value. Lowering the concentration of citrate leads to a lower firing intensity of DRG sensory neurons.

conclusionIncreased activity/firing of DRG sensory neurons has been suggested as a key feature underlying nociception. Our results support the utility of an in vitro MEA assay with cultured DRG sensory neurons to probe the nociceptive potential of clinically relevant buffer components used in injectable biologics.

Indexed as

AnimalsBiological ProductsBuffersCells, CulturedDrug Evaluation, PreclinicalElectrodesGanglia, SpinalInjectionsInjection Site ReactionNociceptionPainPrimary Cell CultureRatsSensory Receptor CellsBiological ProductsBuffersbuffer systeminjection site painmulti/micro electrode arraynociceptionsensory neurons

Identifiers

PMID34244893
OpenAlexW3180835029

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.