ArticleAnalytical and bioanalytical chemistry2022
Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS.
Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Standard Reference Material (SRM®) 1950 metabolites in frozen human plasma-use, impact, lessons learned, and future perspectives.Analytical and bioanalytical chemistry · 2026Review
- Robust analytical methods for bis(monoacylglycero)phosphate profiling in health and disease.Nature protocols · 2026Review
- The Language of Elution: Autoregressive Prediction of the Next Feature in Untargeted LC-HRMS Lipidomics.ArXiv · 2026Article
- Exploration of the structural diversity and distribution pattern of gangliosides and sulfatides in mouse brain tissues and biomarkers for Parkinson's Disease.Lipids in health and disease · 2026Article
- What's in a name? Metabolite identification: challenges and pitfalls in untargeted metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2026Review
- Benzoyl chloride derivatization improves selectivity and sensitivity of lipidomic quantitation in human serum of pancreatic cancer patients using RP-UHPLC/MS/MS.Analytical and bioanalytical chemistry · 2026Article
- Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reversed-Phase UHPSFC/MS/MS.Analytical chemistry · 2025Article
- Benchmarking of Trapped Ion Mobility Spectrometry in Differentiating Plasmalogens from Other Ether Lipids in Lipidomics Experiments.Analytical chemistry · 2025Article
- 2-Ethylhexyl Diphenyl Phosphate Affects Steroidogenesis and Lipidome Profile in Human Adrenal (H295R) Cells.Chemical research in toxicology · 2025Article
- Novel Charge-Switch Derivatization Method Using 3-(Chlorosulfonyl)benzoic Acid for Sensitive RP-UHPLC/MS/MS Analysis of Acylglycerols, Sterols, and Prenols.Analytical chemistry · 2025Article
- Ultrahigh-Performance Supercritical Fluid Chromatography-Mass Spectrometry in the Clinical Lipidomic Analysis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Reversed-Phase Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Method for High-Throughput Lipidomic Quantitation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Four-Dimensional Lipidomic Analysis Using Comprehensive Online UHPLC × UHPSFC/Tandem Mass Spectrometry.Analytical chemistry · 2024Article
- Highly reliable LC-MS lipidomics database for efficient human plasma profiling based on NIST SRM 1950.Journal of lipid research · 2024Article
- LC-ESI-HRMS - lipidomics of phospholipids : Characterization of extraction, chromatography and detection parameters.Analytical and bioanalytical chemistry · 2024Article
- High-Throughput Microbore LC-MS Lipidomics to Investigate APOE Phenotypes.Analytical chemistry · 2024Article
- Analysis of Phospholipids in Digestion Using Hybrid IDA and SWATH Acquisition: An Example for Krill Oil.Foods (Basel, Switzerland) · 2023Article
- Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples.Nature communications · 2023Article
- Lipidomics-Paving the Road towards Better Insight and Precision Medicine in Rare Metabolic Diseases.International journal of molecular sciences · 2023Review
- What if using certified reference materials (CRMs) was a requirement to publish in analytical/bioanalytical chemistry journals?Analytical and bioanalytical chemistry · 2022Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method was developed with the aim to unambiguously identify a large number of lipid species from multiple lipid classes in human plasma. The optimized RP-UHPLC/MS method employed the C18 column with sub-2-μm particles with the total run time of 25 min. The chromatographic resolution was investigated with 42 standards from 18 lipid classes. The UHPLC system was coupled to high-resolution quadrupole-time-of-flight (QTOF) mass analyzer using electrospray ionization (ESI) measuring full-scan and tandem mass spectra (MS/MS) in positive- and negative-ion modes with high mass accuracy. Our identification approach was based on m/z values measured with mass accuracy within 5 ppm tolerance in the full-scan mode, characteristic fragment ions in MS/MS, and regularity in chromatographic retention dependences for individual lipid species, which provides the highest level of confidence for reported identifications of lipid species including regioisomeric and other isobaric forms. The graphs of dependences of retention times on the carbon number or on the number of double bond(s) in fatty acyl chains were constructed to support the identification of lipid species in homologous lipid series. Our list of identified lipid species is also compared with previous publications investigating human blood samples by various MS-based approaches. In total, we have reported more than 500 lipid species representing 26 polar and nonpolar lipid classes detected in NIST Standard reference material 1950 human plasma.
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