ArticleBMC genomics2021
Trehalose in pine wood nematode participates in DJ3 formation and confers resistance to low-temperature stress.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Combined Transcriptomic and Metabolomic Analyses of Low-Temperature Adaptation inInternational journal of molecular sciences · 2026Article
- Enzymes involved in trehalose-chitin synthesis in Haemonchus contortus could be vaccine candidates for goats.Parasites & vectors · 2025Article
- Identification and Transcriptome Analysis ofInternational journal of molecular sciences · 2024Article
- Detection Methods for Pine Wilt Disease: A Comprehensive Review.Plants (Basel, Switzerland) · 2024Review
- Caffeic acid: A game changer in pine wood nematode overwintering survival.Molecular plant pathology · 2024Article
- Evaluating Vacuum and Steam Heat to Eliminate Pinewood Nematodes in Naturally Infested Whole Pine Logs.Journal of nematology · 2024Article
- Differences of Pine Wood Nematode (Biology · 2024Article
- Genetic diversity and genome-wide association analysis of pine wood nematode populations in different regions of China.Frontiers in plant science · 2023Article
- Article
- Population differentiation and epidemic tracking of Bursaphelenchus xylophilus in China based on chromosome-level assembly and whole-genome sequencing data.Pest management science · 2022Article
- Phytochemicals as Biopesticides against the Pinewood NematodePlants (Basel, Switzerland) · 2021Review
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
backgroundRecently, pine wood nematode (PWN, Bursaphelenchus xylophilus) has been found in the extreme cold area of northeast China. The third-stage dispersal juvenile (DJ3) of PWN, which is a long-lived stress-resistant stage, plays an important role in the process of PWN spreading to low-temperature areas, as this stage can survive under unfavorable conditions.
resultsWeighted correlation network analysis (WGCNA) was used to analyze the expression patterns of 15,889 genes included in 21 RNA-Seq results of PWN at DJ3 and the other 6 different stages, and a total of 12 coexpression modules were obtained. Among them, the magenta module has the highest correlation with DJ3, which included a total of 652 genes. KEGG enrichment analysis showed that most of the genes in the magenta module were involved in metabolic processes, which were related to autophagy and longevity regulation. These pathways included starch and sucrose metabolism, which contains trehalose metabolism. To explore the function of trehalose in DJ3 formation and survival under - 20 °C, a trehalose-6-phosphate synthase encoding gene (Bx-tps), a trehalose-6-phosphate phosphatase encoding gene (Bx-tpp) and 7 trehalase encoding genes (Bx-tres) were identified and investigated. The expression of these 9 genes was related to the formation of DJ3. A treatment under - 20 °C induced the accumulation of trehalose. The survival rate of DJ3 at -20 °C reduced after silencing of any of these trehalose metabolism genes. Further analysis suggested that two trehalose synthesis genes were highly correlated with DJ3 and might be involved in autophagy by regulating with energy conversion related genes.
conclusionsThe above results indicated that trehalose metabolism promotes DJ3 formation and helps DJ3 survive at -20 °C. Although trehalose accumulation is favorable for DJ3 to cope with low-temperature stress, multiple trehalose metabolism genes need to work together. There may be a multi-path regulated physiological process involving trehalose synthesis genes under low-temperature stress resistance. This physiological process may regulate the formation and maintenance of DJ3 through autophagy and energy conversion.
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