ArticleOptica2021
3D super-resolution deep-tissue imaging in living mice.
Article in Optica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 75 citations in OpenAlex.
- Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.Biomedical optics express · 2026Article
- High-fidelity intravital imaging of biological dynamics with latent-space-enhanced digital adaptive optics.Nature biotechnology · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Descattering and image restoration with a transformer-based neural network in deep tissue imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- In situ fully vectorial tomography and pupil function retrieval of tightly focused fields.Nature communications · 2025Article
- Microscopy methods for the in vivo study of nanoscale nuclear organization.Biochemical Society transactions · 2025Review
- Super-resolution imaging of fast morphological dynamics of neurons in behaving animals.Nature methods · 2025Article
- Adaptive optics in an oblique plane microscope.Biomedical optics express · 2024Article
- Suppression of Subpixel Jitter in Resonant Scanning Systems With Phase-locked Sampling.IEEE transactions on medical imaging · 2024Article
- Selective-plane-activation structured illumination microscopy.Nature methods · 2024Article
- Adaptive Optics in an Oblique Plane Microscope.bioRxiv : the preprint server for biology · 2024Article
- Impact of a tilted coverslip on two-photon and STED microscopy.Biomedical optics express · 2024Article
- Understanding the nervous system: lessons from Frontiers in Neurophotonics.Neurophotonics · 2024Review
- Optimal real-time resonant scanner linearization using filtered Hermite interpolation.Biomedical optics express · 2023Article
- Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy.Neurophotonics · 2023Article
- Dense 4D nanoscale reconstruction of living brain tissue.Nature methods · 2023Article
- Article
- Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time.Frontiers in immunology · 2023Review
- Super-Resolution Microscopy Opens New Doors to Life at the Nanoscale.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Review
- The Beauty and Complexity of Blood Vessel Patterning.Cold Spring Harbor perspectives in medicine · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.