Evidence map›Paper›PMID 34239605›Full record

ArticleCardiovascular therapeutics2021

Patients with Coronary Artery Disease Have Lower Levels of Antibody to Heat-Stressed Fibroblast Derived Proteins, versus Normal Subjects.

Azin Aghamajidi, Hesam Babaei Khameneh, Narges Amirjamshidi, Seyed Farzad Jalali, Haleh Akhavan-Niaki, Soraya Khafri, Seyedeh Narges Mousavi, Monireh Golpour, Maryam Mehri, AmrollahMostafazadeh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular therapeutics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Azin AghamajidiStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0001-5904-5260
Hesam Babaei KhamenehStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0002-4657-2448
Narges AmirjamshidiStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Seyed Farzad JalaliDepartment of Cardiology, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Haleh Akhavan-NiakiCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0003-3681-0227
Soraya KhafriDepartment of Social Medicine, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0002-2398-7560
Seyedeh Narges MousaviCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Monireh GolpourMolecular and Cell Biology Research Center, Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Science, Sari, Iran.ORCID https://orcid.org/0000-0003-1406-9028
Maryam MehriStudents Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0003-2208-6186
AmrollahMostafazadehCellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.ORCID https://orcid.org/0000-0003-3178-9203

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cellular stress response plays an important role in the pathophysiology of coronary artery disease (CAD). Inhibition of cellular stress may provide a novel clinical approach regarding the diagnosis and treatment of CAD. Fibroblasts constitute 60-70% of cardiac cells and have a crucial role in cardiovascular function. Hence, the aim of this study was to show a potential therapeutic application of proteins derived from heat-stressed fibroblast in CAD patients. Fibroblasts were isolated from the foreskin and cultured under heat stress conditions. Surprisingly, 1.06% of the cells exhibited a necrotic death pattern. Furthermore, heat-stressed fibroblasts produced higher level of total proteins than control cells. In SDS-PAGE analysis, a 70 kDa protein band was observed in stressed cell culture supernatants which appeared as two acidic spots with close pI in the two-dimensional electrophoresis. To evaluate the immunogenic properties of fibroblast-derived heat shock proteins (HSPs), the serum immunoglobulin-G (IgG) was measured by ELISA in 50 CAD patients and 50 normal subjects who had been diagnosed through angiography. Interestingly, the level of anti-HSP antibody was significantly higher in non-CAD individuals in comparison with the patient's group (

Indexed as

AgedCoronary Artery DiseaseEnzyme-Linked Immunosorbent AssayFemaleFibroblastsHeat-Shock ProteinsHeat-Shock ResponseHumansImmunoglobulin GMaleMiddle AgedOdds RatioHeat-Shock ProteinsImmunoglobulin G

Identifiers

PMID34239605
PMCPMC8225444

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.