Evidence map›Paper›PMID 34239574›Full record

ArticleStem cells international2021

Influence of Dental Pulp Harvesting Method on the Viability and Differentiation Capacity of Adult Dental Pulp-Derived Mesenchymal Stem Cells.

Victor Okada Vendramini, Sevda Pouraghaei, Rafael Maza Barbosa, Antônio Carlos Aloise, José Ricardo Ferreira Muniz, Marcelo Sperandio, Peter Karyen Moy, André Antonio Pelegrine, Alireza Moshaverinia

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Victor Okada VendraminiDepartment of Implant Dentistry, Faculdade São Leopoldo Mandic, Rua Areias, 37, Campinas, São Paulo 13024-530, Brazil.
Sevda PouraghaeiDepartment of Advanced Prosthodontics, University of California at Los Angeles, School of Dentistry, Los Angeles, USA.
Rafael Maza BarbosaRCrio, Campinas, Brazil.
Antônio Carlos AloiseDepartment of Implant Dentistry, Faculdade São Leopoldo Mandic, Rua Areias, 37, Campinas, São Paulo 13024-530, Brazil.
José Ricardo Ferreira MunizRCrio, Campinas, Brazil.
Marcelo SperandioDepartment of Oral Pathology, Faculdade São Leopoldo Mandic, Campinas, Brazil.
Peter Karyen MoyDepartment of Oral & Maxillofacial Surgery, University of California at Los Angeles, School of Dentistry, Los Angeles, USA.
André Antonio PelegrineDepartment of Implant Dentistry, Faculdade São Leopoldo Mandic, Rua Areias, 37, Campinas, São Paulo 13024-530, Brazil.ORCID https://orcid.org/0000-0001-7935-1062
Alireza MoshaveriniaDepartment of Advanced Prosthodontics, University of California at Los Angeles, School of Dentistry, Los Angeles, USA.ORCID https://orcid.org/0000-0002-1542-6202
Faculdade São Leopoldo Mandic · BRUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo compare two pulp harvesting methods for stem cell expansion, namely, conservative pulpotomy and pulpectomy from exodontia.

methodTen freshly extracted sound third molars from five patients were selected. Five were used in the control group, where pulp harvesting was performed by exodontia and the remaining teeth were used in the test group, where the pulp was harvested by conservative pulpotomy (preserving the tooth). This was a split-mouth design study, where a third molar from one side was randomly allocated into the test group and the contralateral tooth in the control group. After pulp harvesting, the following evaluations were performed: cell morphology, sterility test, immunophenotyping, differentiation assays, first pass live cell counts, time to cryopreservation, and total number of expanded cells at the end of the fourth pass.

resultsRegarding morphology, the cells from both groups presented a fibroblastic phenotype. All samples were sterile. Immunophenotyping demonstrated a positive expression for CD105, CD90, and CD73 and negative expression for CD45 in both groups. Differentiation assays were positive for osteogenic and chondrogenic differentiation in both groups. Regarding live cell counts in the first passage, the control group had 95.8% live cells in the total count and the test group 91.2% (

conclusionThese results suggest that adult stem cell harvesting from conservative pulpotomy is as effective as the traditional exodontia-based method.

Identifiers

PMID34239574
PMCPMC8238610
OpenAlexW3174687687

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.