Evidence map›Paper›PMID 34238995›Full record

ArticleScientific reports2021

Screening of the HBx transactivation domain interacting proteins and the function of interactor Pin1 in HBV replication.

Qiaoxia Zhou, Libo Yan, Baofu Xu, Xue'er Wang, Xuehong Sun, Ning Han, Hong Tang, Feijun Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Identifying potential monkeypox virus inhibitors: anFrontiers in cellular and infection microbiology · 2024
    Article
  6. Relevance of HBx for Hepatitis B Virus-Associated Pathogenesis.International journal of molecular sciences · 2023
    Review
  7. Roles of peptidyl prolyl isomerase Pin1 in viral propagation.Frontiers in cell and developmental biology · 2022
    Review
  8. Review
  9. SPD_0090 Negatively Contributes to Virulence ofFrontiers in microbiology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Qiaoxia Zhou *Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Third Renmin Road North, Chengdu, 610041, People's Republic of China.
Libo Yan *Center of Infectious Diseases, West China Hospital of Sichuan University, No.37 Guo Xue Xiang, Chengdu, 610041, People's Republic of China.
Baofu XuDepartment of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Third Renmin Road North, Chengdu, 610041, People's Republic of China.
Xue'er WangDepartment of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Third Renmin Road North, Chengdu, 610041, People's Republic of China.
Xuehong SunDepartment of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Third Renmin Road North, Chengdu, 610041, People's Republic of China.
Ning HanCenter of Infectious Diseases, West China Hospital of Sichuan University, No.37 Guo Xue Xiang, Chengdu, 610041, People's Republic of China.
Hong TangCenter of Infectious Diseases, West China Hospital of Sichuan University, No.37 Guo Xue Xiang, Chengdu, 610041, People's Republic of China. htang6198@hotmail.com.
Feijun HuangDepartment of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Third Renmin Road North, Chengdu, 610041, People's Republic of China. fjhuang60123@hotmail.com.
Sichuan University · CNState Key Laboratory of BiotherapyXinxiang Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatitis B virus (HBV) X protein (HBx) has been determined to play a crucial role in the replication and transcription of HBV, and its biological functions mainly depend on the interaction with other host proteins. This study aims at screening the proteins that bind to the key functional domain of HBx by integrated proteomics. Proteins that specifically bind to the transactivation domain of HBx were selected by comparing interactors of full-length HBx and HBx-D5 truncation determined by glutathione-S-transferase (GST) pull-down assay combined with mass spectrometry (MS). The function of HBx interactor Pin1 in HBV replication was further investigated by in vitro experiments. In this study, a total of 189 proteins were identified from HepG2 cells that specifically bind to the transactivation domain of HBx by GST pull-down and subsequent MS. After gene ontology (GO) analysis, Pin1 was selected as the protein with the highest score in the largest cluster functioning in protein binding, and also classified into the cluster of proteins with the function of structural molecule activity, which is of great potential to be involved in HBV life cycle. The interaction between Pin1 and HBx has been further confirmed by Ni

Indexed as

Transcriptional ActivationHepatitis B virusHep G2 CellsHumansNIMA-Interacting Peptidylprolyl IsomeraseProtein BindingProtein DomainsProtein Interaction MapsTrans-ActivatorsViral Regulatory and Accessory ProteinsVirus ReplicationWnt Signaling Pathwayhepatitis B virus X proteinNIMA-Interacting Peptidylprolyl IsomerasePIN1 protein, humanTrans-ActivatorsViral Regulatory and Accessory Proteins

Identifiers

PMID34238995
PMCPMC8266847
OpenAlexW3179362069

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.