ArticleScientific reports2021
Molecular detection and phylogenetic analyses of Anaplasma spp. in Haemaphysalis longicornis from goats in four provinces of China.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 22 citations in OpenAlex.
- Epidemiology and genetic evolution of Anaplasma species in rodents from southeastern China.Scientific reports · 2026Article
- Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR.Veterinary research communications · 2026Article
- Epidemiological Investigation and Phylogenetic Analysis of Major Blood-Derived Pathogens in Sheep from Gansu Province.Pathogens (Basel, Switzerland) · 2026Article
- Molecular detection and genetic diversity ofFrontiers in microbiology · 2026Article
- Molecular characterization of tick-borne bacterial and protozoan pathogens in parasitic ticks from Xinjiang, China.Parasites & vectors · 2025Article
- Molecular Prevalence and Phylogenetic Analysis ofVeterinary sciences · 2025Article
- Molecular Detection and Phylogenetic Analysis ofVeterinary sciences · 2025Article
- Prevalence and genetic diversity of Anaplasma and Ehrlichia in ticks and domesticated animals in Suizhou County, Hubei Province, China.Scientific reports · 2024Article
- Anaplasma capra: a new emerging tick-borne zoonotic pathogen.Veterinary research communications · 2024Review
- Identification of Bacterial Communities and Tick-Borne Pathogens inTropical medicine and infectious disease · 2022Article
- A Quantum Vaccinomics Approach for the Design and Production of MSP4 Chimeric Antigen for the Control ofVaccines · 2022Article
- Co-existence of MultipleFrontiers in microbiology · 2022Article
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10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anaplasma species, which are distributed worldwide, are gram-negative obligate intracellular tick-borne bacteria that pose a threat to human and animal health. Haemaphysalis longicornis ticks play a vital role as vectors in the transmission of Anaplasma pathogens. However, the Anaplasma species carried by H. longicornis in China are yet to be characterized. In this study, 1074 H. longicornis specimens were collected from goats in four provinces of China from 2018 to 2019 and divided into 371 sample pools. All tick sample pools were examined for the presence of Anaplasma species via nested PCR amplification of 16S ribosomal RNA, major surface protein 4 (msp4), or citric acid synthase (gltA) genes, which were sequenced to determine the molecular and phylogenetic characteristics of the isolates. The overall Anaplasma spp-positive rate of H. longicornis was determined to be 26.68% (99/371). The percentage prevalence of A. phagocytophilum-like1, A. bovis, A. ovis, A. marginale, and A. capra were 1.08% (4/371), 13.21% (49/371), 13.21% (49/371), 1.35% (5/371), and 10.24% (38/371), respectively, and the co-infection rate of two or more types of Anaplasma was 6.47% (24/371). Phylogenetic analyses led to the classification of A. phagocytophilum into an A. phagocytophilum-like1 (Anaplasma sp. Japan) group. Anaplasma bovis sequences obtained in this study were 99.8-100% identical to those of an earlier strain isolated from a Chinese tick (GenBank accession no. KP314251). Anaplasma ovis sequences showed 99.3-99.6% identity to an A. ovis human strain identified from a Cypriot patient (GenBank accession no. FJ460443). Only one msp4 sequence of A. marginale was detected and was grouped with those of other A. marginale isolates, and these A. capra isolates obtained in this present study may be zoonotic. The detection and characterization of four Anaplasma species in H. longicornis in this study have added to the current knowledge of the parasite and provided data on multiple Anaplasma species with veterinary and medical significance from four provinces of China.
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