Evidence map›Paper›PMID 34238213›Full record

ArticleCellular & molecular biology letters2021

LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis.

An Yang, Xin Liu, Ping Liu, Yunzhang Feng, Hongbo Liu, Shen Gao, Limin Huo, Xinyan Han, Jurong Wang, Wei Kong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular & molecular biology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 57 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

An YangHandan Central Hospital, Handan, 056001, Hebei Province, China.
Xin LiuAffiliated Hospital of Hebei University of Engineering, Handan, 056002, Hebei Province, China.
Ping LiuHandan Central Hospital, Handan, 056001, Hebei Province, China.
Yunzhang FengHandan Central Hospital, Handan, 056001, Hebei Province, China.
Hongbo LiuHandan Central Hospital, Handan, 056001, Hebei Province, China.
Shen GaoHandan First Hospital, Handan, 056002, Hebei Province, China.
Limin HuoHandan First Hospital, Handan, 056002, Hebei Province, China.
Xinyan HanHandan Central Hospital, Handan, 056001, Hebei Province, China.
Jurong WangHandan Central Hospital, Handan, 056001, Hebei Province, China.
Wei KongHandan Central Hospital, Handan, 056001, Hebei Province, China. kongwei78@163.com.ORCID http://orcid.org/0000-0003-1977-6127
Handan College · CNHebei University of Engineering · CN

Funding

Hebei Provincial Department of Science and Technology 162777258
6 · The paper itself

Abstract

backgroundLong noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we aimed to identify how UCA1 promotes gastric cancer development.

methodsThe Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to analyze UCA1 and myosin VI (MYO6) expression in gastric cancer. Western blot and quantitative real-time PCR (QPCR) were performed to test the expression level of the UCA1/miR-145/MYO6 axis in gastric cancer cell lines and tissues. The roles of the UCA1/miR-145/MYO6 axis in gastric cancer in vitro and in vivo were investigated by CCK-8 assay, flow cytometry, siRNAs, immunohistochemistry, and a mouse xenograft model. The targeted relationship among UCA1, miR-145, and MYO6 was predicted using LncBase Predicted v.2 and TargetScan online software, and then verified by luciferase activity assay and RNA immunoprecipitation.

resultsUCA1 expression was higher but miR-145 expression was lower in gastric cancer cell lines or tissues, compared to the adjacent normal cell line or normal tissues. Function analysis verified that UCA1 promoted cell proliferation and inhibited cell apoptosis in the gastric cancer cells in vitro and in vivo. Mechanistically, UCA1 could bind directly to miR-145, and MYO6 was found to be a downstream target gene of miR-145. miR-145 mimics or MYO6 siRNAs could partly reverse the effect of UCA1 on gastric cancer cells.

conclusionsUCA1 accelerated cell proliferation and inhibited cell apoptosis through sponging miR-145 to upregulate MYO6 expression in gastric cancer, indicating that the UCA1/miR-145/MYO6 axis may serve as a potential therapeutic target for gastric cancer.

Indexed as

AnimalsApoptosisBiomarkers, TumorCell Line, TumorCell ProliferationComputational BiologyDatabases, GeneticHumansMaleMiceMice, Inbred BALB CMice, NudeMicroRNAsMiddle AgedMyosin Heavy ChainsRNA, Long NoncodingBiomarkers, TumorMicroRNAsMIRN145 microRNA, humanMyosin Heavy Chainsmyosin VIRNA, Long NoncodingUCA1 RNA, humanGastric cancerlncRNAmiR-145MYO6UCA1

Identifiers

PMID34238213
PMCPMC8268585
OpenAlexW3178233070

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.