ArticlePLoS genetics2021
Transcriptomic and epigenomic profiling of young and aged spermatogonial stem cells reveals molecular targets regulating differentiation.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 22 citations in OpenAlex.
- Normal spermatogenesis in older men is associated with compensatory transcriptome changes.GeroScience · 2026Article
- SIRT7 links H3K36ac epigenetic regulation with genome maintenance in the mouse testis.Nature communications · 2026Article
- Effects of Aging on Mammalian Spermatogenesis From a Cellular Perspective.Molecular reproduction and development · 2025Review
- Cellular and molecular functions of long noncoding RNAs in testis, aging and diseases.Biogerontology · 2025Review
- Exploring the Mechanisms of Testicular Aging: Advances in Biomarker Research.Aging and disease · 2025Review
- Histone lysine methyltransferases and their specific methylation marks show significant changes in mouse testes from young to older ages.Biogerontology · 2025Article
- Male aging in germ cells: What are we inheriting?Genetics and molecular biology · 2025Article
- The Biology and Regulation of Spermatogonial Stem Cells in the Niche.Advances in experimental medicine and biology · 2025Review
- Single-cell transcriptome profiling highlights the importance of telocyte, kallikrein genes, and alternative splicing in mouse testes aging.Scientific reports · 2024Article
- Reproductive Ageing: Current insights and a potential role of NAD in the reproductive health of aging fathers and their children.Reproduction (Cambridge, England) · 2024Review
- Cell-type specific and differential expression of LINC-RSAS long noncoding RNA declines in the testes during ageing of the rat.Biogerontology · 2024Article
- AgeAnnoMO: a knowledgebase of multi-omics annotation for animal aging.Nucleic acids research · 2024Article
- A single-cell transcriptomic landscape of mouse testicular aging.Journal of advanced research · 2023Article
- Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro.International journal of molecular sciences · 2023Article
- Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole (Zoological research · 2022Article
- Review
- Testicular aging, male fertility and beyond.Frontiers in endocrinology · 2022Review
- Editorial: Germ cell development and reproductive aging.Frontiers in cell and developmental biology · 2022Article
- Article
- KEAP1-NRF2 system regulates age-related spermatogenesis dysfunction.Reproductive medicine and biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermatogonial stem cells (SSC), the foundation of spermatogenesis and male fertility, possess lifelong self-renewal activity. Aging leads to the decline in stem cell function and increased risk of paternal age-related genetic diseases. In the present study, we performed a comparative genomic analysis of mouse SSC-enriched undifferentiated spermatogonia (Oct4-GFP+/KIT-) and differentiating progenitors (Oct4-GFP+/KIT+) isolated from young and aged testes. Our transcriptome data revealed enormous complexity of expressed coding and non-coding RNAs and alternative splicing regulation during SSC differentiation. Further comparison between young and aged undifferentiated spermatogonia suggested these differentiation programs were affected by aging. We identified aberrant expression of genes associated with meiosis and TGF-β signaling, alteration in alternative splicing regulation and differential expression of specific lncRNAs such as Fendrr. Epigenetic profiling revealed reduced H3K27me3 deposition at numerous pro-differentiation genes during SSC differentiation as well as aberrant H3K27me3 distribution at genes in Wnt and TGF-β signaling upon aging. Finally, aged undifferentiated spermatogonia exhibited gene body hypomethylation, which is accompanied by an elevated 5hmC level. We believe this in-depth molecular analysis will serve as a reference for future analysis of SSC aging.
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