Evidence map›Paper›PMID 34236771›Full record

ArticleChemMedChem2021

Fluorescent Amino Acid Initiated de novo Cyclic Peptides for the Label-Free Assessment of Cell Permeability*.

Yuteng Wu, M Teresa Bertran, James Rowley, Ewen D D Calder, Dhira Joshi, Louise J Walport

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemMedChem, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Anion-Sensing Properties of Cyclopentaphenylalanine.Molecules (Basel, Switzerland) · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuteng WuProtein-Protein Interaction Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
M Teresa BertranProtein-Protein Interaction Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
James RowleyProtein-Protein Interaction Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
Ewen D D CalderProtein-Protein Interaction Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
Dhira JoshiPeptide Chemistry, The Francis Crick Institute, London, NW1 1AT, UK.
Louise J WalportProtein-Protein Interaction Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.ORCID 0000-0002-3795-8365

Funding

Wellcome Trust FC001748
6 · The paper itself

Abstract

The major obstacle in applying peptides to intracellular targets is their low inherent cell permeability. Standard approaches to attach a fluorophore (e. g. FITC, TAMRA) can change the physicochemical properties of the parent peptide and influence their ability to penetrate and localize in cells. We report a label-free strategy for evaluating the cell permeability of cyclic peptide leads. Fluorescent tryptophan analogues 4-cyanotryptophan (4CNW) and β-(1-azulenyl)-L-alanine (AzAla) were incorporated into in vitro translated macrocyclic peptides by initiator reprogramming. We then demonstrate these efficient blue fluorescent emitters are good tools for monitoring peptide penetration into cells.

Indexed as

Optical ImagingAlanineAzulenesCell Line, TumorFluorescent DyesHumansMolecular StructurePeptides, CyclicPermeabilitySesquiterpenesTryptophan4-cyanotryptophanacetyl-(1-azulenyl)-alanineAlanineAzulenesFluorescent DyesPeptides, CyclicSesquiterpenesTryptophancell permeabilityfluorescence imaginglabel-freepeptidesRaPID

Identifiers

PMID34236771
PMCPMC8597039

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.