Evidence map›Paper›PMID 34236124›Full record

ReviewNeurogastroenterology and motility2022

The gut brain in a dish: Murine primary enteric nervous system cell cultures.

Simone L Schonkeren, Tara T Küthe, Musa Idris, Ana C Bon-Frauches, Werend Boesmans, Veerle Melotte

Open access · bronzeAbstract readReview
In one paragraph

Review in Neurogastroenterology and motility, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Rodent Enteric Glia Culture.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  3. Article
  4. Article
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  6. Article
  7. Review
  8. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Simone L SchonkerenDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.ORCID 0000-0003-4715-4173
Tara T KütheDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.
Musa IdrisDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.
Ana C Bon-FrauchesDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.ORCID 0000-0001-7325-6021
Werend BoesmansDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.ORCID 0000-0002-2426-0451
Veerle MelotteDepartment of Pathology, Maastricht University Medical Center, Maastricht, Netherlands.ORCID 0000-0002-9459-123X
Maastricht University Medical Centre · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. PURPOSE: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.

Indexed as

Enteric Nervous SystemAnimalsBrainCell Culture TechniquesMiceNeurogliaNeuronsadult mouseenteric glial cellsenteric neuronsprimary ENS cultureprotocol

Identifiers

PMID34236124
PMCPMC9285479
OpenAlexW3182861767

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.