Evidence map›Paper›PMID 34235651›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Purification of Hibernating and Active C- Ribosomes from Zinc-Starved Mycobacteria.

Yunlong Li, Pooja Keshavan, Jamie H Corro, Ravi K Koripella, Rajendra K Agrawal, Anil K Ojha

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Ribosome hibernation in zinc-starvedMicrobiology spectrum · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yunlong LiDivision of Genetics, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Pooja KeshavanDivision of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Jamie H CorroDivision of Genetics, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Ravi K KoripellaDivision of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Rajendra K AgrawalDivision of Translational Medicine, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Anil K OjhaDivision of Genetics, Wadsworth Center, New York State Department of Health, Albany, NY, USA. anil.ojha@health.ny.gov.
New York State Department of Health · USUniversity at Albany, State University of New York · USWadsworth Center · US

Funding

Structural Aspects of Protein Synthesis in the Mammalian MitochondriaR01GM061576 · NIGMS · WADSWORTH CENTER · PI AGRAWAL, RAJENDRA K · 2001 to 2018
$6.4M
Alternative Ribosomes & Antibiotic Tolerance in MycobacteriaR01AI132422 · NIAID · WADSWORTH CENTER · PI RAJENDRA K AGRAWAL, Anil Kumar Ojha · 2017 to 2026
$3.8M
Physiology of Mycobacterium Tuberculosis with Hibernating RibosomesR21AI144474 · NIAID · WADSWORTH CENTER · PI OJHA, ANIL KUMAR · 2019 to 2020
$466k
NIAID NIH HHS R01 AI132422NIAID NIH HHS R21 AI144474NIGMS NIH HHS R01 GM061576
6 · The paper itself

Abstract

Zinc starvation in Mycobacterium smegmatis and Mycobacterium tuberculosis induces ribosome remodeling and hibernation. Remodeling involves replacement of C+ ribosomal (r-) proteins containing the zinc-binding CXXC motif with their C- paralogues without the motif. Hibernation is characterized by binding of mycobacterial-specific protein Y (Mpy) to 70S C- ribosomes, stabilizing the ribosome in an inactive state that is also resistant to kanamycin and streptomycin. We observed that ribosome remodeling and hibernation occur at two different concentrations of cellular zinc. Here, we describe the methods to purify hibernating and active forms of C- ribosomes from zinc-starved mycobacteria, along with purification of C+ ribosomes from zinc-rich mycobacterial cells. In vitro analysis of these distinct types of ribosomes will facilitate screening of small molecule inhibitors of ribosome hibernation for improved therapeutics against mycobacterial infections.

Indexed as

Bacterial ProteinsDrug Resistance, BacterialMycobacterium smegmatisMycobacterium tuberculosisPolyribosomesRibosomal ProteinsRibosomesRNA, RibosomalZincBacterial ProteinsRibosomal ProteinsRNA, RibosomalZincDormancyDug resistanceMycobacterial persistence

Identifiers

PMID34235651
PMCPMC9202777
OpenAlexW3181875588

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.