ArticleMethods in molecular biology (Clifton, N.J.)2021
Lipid and Lipoarabinomannan Isolation and Characterization.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A single mycobacterial ligand organizes multi-receptor signaling to reprogram macrophage lipid metabolism.bioRxiv : the preprint server for biology · 2026Article
- Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex.Nature communications · 2023Article
- Contraction and expansion dynamics: deciphering genomic underpinnings of growth rate and pathogenicity inFrontiers in microbiology · 2023Article
- LysX2 is a Mycobacterium tuberculosis membrane protein with an extracytoplasmic MprF-like domain.BMC microbiology · 2022Article
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Authors and funding
5 authors.
Funding
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Abstract
The very high content of structurally diverse and biologically active lipids of exotic structures is the hallmark of Mycobacteria. As such the lipid composition is commonly used to characterize mycobacterial strains at the species and type-species levels. The present chapter describes the methods that allow the purification of the most commonly isolated biologically active lipids and those used for analyzing extractable lipids and their constituents, cell wall-linked mycolic acids (MA), and lipoarabinomannan (LAM). These involve various chromatographic techniques and analytical procedures necessary for structural and metabolic studies of mycobacterial lipids. In addition, as the use of physical methods has brought important overhang on chemical structures of the very-long-chain MA, which typify mycobacteria, NMR and mass spectrometry data of these specific fatty acids are included.
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Registered trials
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