Evidence map›Paper›PMID 34234774›Full record

ArticleFrontiers in immunology2021

Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey.

Leslie Y T Leung, Srijit Khan, Patrick Budylowski, Zhijie Li, Sofiya Goroshko, Yanling Liu, Shilan Dong, James R Carlyle, James M Rini, Mario Ostrowski and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Leslie Y T LeungDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Srijit KhanDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Patrick BudylowskiDepartment of Medicine, University of Toronto, Toronto, ON, Canada.
Zhijie LiDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
Sofiya GoroshkoDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Yanling LiuDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Shilan DongDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
James R CarlyleDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
James M RiniDepartment of Biochemistry, University of Toronto, Toronto, ON, Canada.
Mario OstrowskiDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
Götz R A EhrhardtDepartment of Immunology, University of Toronto, Toronto, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SARS-CoV-2 is a newly emerged betacoronavirus and the causative agent for the COVID-19 pandemic. Antibodies recognizing the viral spike protein are instrumental in natural and vaccine-induced immune responses to the pathogen and in clinical diagnostic and therapeutic applications. Unlike conventional immunoglobulins, the variable lymphocyte receptor antibodies of jawless vertebrates are structurally distinct, indicating that they may recognize different epitopes. Here we report the isolation of monoclonal variable lymphocyte receptor antibodies from immunized sea lamprey larvae that recognize the spike protein of SARS-CoV-2 but not of other coronaviruses. We further demonstrate that these monoclonal variable lymphocyte receptor antibodies can efficiently neutralize the virus and form the basis of a rapid, single step SARS-CoV-2 detection system. This study provides evidence for monoclonal variable lymphocyte receptor antibodies as unique biomedical research and potential clinical diagnostic reagents targeting SARS-CoV-2.

Indexed as

AnimalsAntibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralBiological EvolutionCOVID-19Cross ReactionsEpitopes, B-LymphocyteFish ProteinsHumansPetromyzonSARS-CoV-2Antibodies, MonoclonalAntibodies, NeutralizingAntibodies, ViralEpitopes, B-LymphocyteFish Proteinsmonoclonal antibodyneutralizationSARS-CoV-2sea lamprey (Petromyzon marinus)variable lymphocyte receptor

Identifiers

PMID34234774
PMCPMC8256154

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.