ArticleStem cells and cloning : advances and applications2021
Isolation and Characterization of Chemo-Resistant Stem Cells from a Mouse Model of Hereditary Non-Polyposis Colon Cancer.
Article in Stem cells and cloning : advances and applications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 5 citations in OpenAlex.
- Comprehensive review of drug resistance in mammalian cancer stem cells: implications for cancer therapy.Cancer cell international · 2024Review
- Review
- Review
- Stem Cell Models for Breast and Colon Cancer: Experimental Approach for Drug Discovery.International journal of molecular sciences · 2022Review
- Stem Cell Models for Cancer Therapy.International journal of molecular sciences · 2022Review
- Drug-Resistant Stem Cells: Novel Approach for Colon Cancer Therapy.International journal of molecular sciences · 2022Review
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1 author.
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Abstract
rationaleLoss of function mutations in DNA mismatch repair genes is the primary genetic defects in high-risk hereditary non-polyposis colon cancer (HNPCC). Cytotoxic chemotherapy and anti-inflammatory drugs are potential treatment options. These treatment options lead to systemic toxicity, acquired tumor resistance and the emergence of drug-resistant stem cells. A colonic epithelial cell culture model expressing the relevant genetic defects in chemo-resistant stem cells provides a relevant experimental system for HNPCC.
objectiveTo develop a colonic epithelial cell culture system from a mouse model for HNPCC and to isolate and characterize drug-resistant stem cells. EXPERIMENTAL MODELS AND BIOMARKERS: The Mlh
resultsThe HNPCC model exhibits aneuploidy, hyper-proliferation, accelerated cell cycle progression and downregulated cellular apoptosis. Long-term exposure to 5-FU selects for the drug-resistant phenotype. These resistant cells exhibit increased formation of tumor spheroids and upregulated expression of cancer stem cell markers CD44, CD133 and c-Myc.
conclusionIn the present study, a stem cell model for HNPCC was validated and offered a novel experimental approach to test stem cell-targeted alternatives to drug-resistant therapy.
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