ArticlePloS one2021
Scanning electron microscopy preparation of the cellular actin cortex: A quantitative comparison between critical point drying and hexamethyldisilazane drying.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 22 citations in OpenAlex.
- The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics.Nature communications · 2026Article
- An Improved SEM Preparation Workflow for Plasma Membrane Imaging in HepG2 and IM-9 Cells.Bioengineering (Basel, Switzerland) · 2026Article
- Few-Layered Conductive Graphene Foams for Electrical Transdifferentiation of Mesenchymal Stem Cells Into Schwann Cell-Like Phenotypes.Advanced healthcare materials · 2026Article
- Multicolor cathodoluminescence imaging of single lanthanide nanoparticles.Nature communications · 2025Article
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- The structure and mechanics of the cell cortex depend on the location and adhesion state.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Self-Assembled Hydrogel Membranes with Structurally Tunable Mechanical and Biological Properties.Biomacromolecules · 2024Article
- Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation.Biotechnology for biofuels and bioproducts · 2023Article
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- Engineering cryoelectrospun elastin-alginate scaffolds to serve as stromal extracellular matrices.Biofabrication · 2022Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular cortex is an approximately 200-nm-thick actin network that lies just beneath the cell membrane. It is responsible for the mechanical properties of cells, and as such, it is involved in many cellular processes, including cell migration and cellular interactions with the environment. To develop a clear view of this dense structure, high-resolution imaging is essential. As one such technique, electron microscopy, involves complex sample preparation procedures. The final drying of these samples has significant influence on potential artifacts, like cell shrinkage and the formation of artifactual holes in the actin cortex. In this study, we compared the three most used final sample drying procedures: critical-point drying (CPD), CPD with lens tissue (CPD-LT), and hexamethyldisilazane drying. We show that both hexamethyldisilazane and CPD-LT lead to fewer artifactual mesh holes within the actin cortex than CPD. Moreover, CPD-LT leads to significant reduction in cell height compared to hexamethyldisilazane and CPD. We conclude that the final drying procedure should be chosen according to the reduction in cell height, and so CPD-LT, or according to the spatial separation of the single layers of the actin cortex, and so hexamethyldisilazane.
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