ArticleScientific reports2021
Fecal sample collection methods and time of day impact microbiome composition and short chain fatty acid concentrations.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Fecal microbiota transplantation from psychiatric patients to mice - systematic review of methodologies and a call for standardization.Translational psychiatry · 2026Pooled it
- The Relationship Between Gut Microbiota, Muscle Mass and Physical Function in Older Individuals: A Systematic Review.Nutrients · 2024Pooled it
- Detection of changes in regional colonic fermentation in response to supplementing a low FODMAP diet with dietary fibres by hydrogen concentrations, but not by luminal pH.Alimentary pharmacology & therapeutics · 2023Trial
- Trial
- Use of Fecal Sampling for Assessing Probiotic Effects on the Gut Microbiome: A Focused Narrative Review.Cureus · 2026Review
- Wheat Peptides Promote Recovery of the Enteric Nervous System via mAChR Activation to Relieve Constipation in Mice.ACS omega · 2026Article
- Oral-gut microbiome dysbiosis in obese smokers reveals compartment-specific shifts.AMB Express · 2026Article
- Basic Microbiome Analysis: Analytical Steps from Sampling to Sequencing.Microorganisms · 2026Review
- Standardized and batch effect-independent technologies enable global collaboration in microbiome research.The ISME journal · 2026Article
- Acromegaly and colorectal cancer risk: emerging roles of the gut microbiota.Frontiers in endocrinology · 2026Review
- Associations between gut microbiome and 24-hour blood pressure variability: a cross-sectional study highlighting sex differences and potential therapeutic targets.Gut microbiome (Cambridge, England) · 2026Article
- Training load influences gut microbiome of highly trained rowing athletes.Journal of the International Society of Sports Nutrition · 2025Article
- Review
- Effects ofMicroorganisms · 2025Article
- Hypertensive disorders of pregnancy were more strongly associated with the functional and network profiles than with the community composition of the gut bacteriome: a pilot study.BMC pregnancy and childbirth · 2025Article
- Comparative study of sample storage conditions on gut dysbiosis in peripheral artery disease.Research square · 2025Article
- Subspecific variation in gut microbiota of North American bison in a sympatric setting reveals differentially abundant taxa.Animal microbiome · 2025Article
- Fecal microbiomes from screening sampling tubes are stable despite varying sampling and storage conditions.Scientific reports · 2025Article
- Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro.Scientific reports · 2025Article
- Diet-Gut Microbiota Relations: Critical Appraisal of Evidence From Studies Using Metagenomics.Nutrition reviews · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Associations between the human gut microbiome and health outcomes continues to be of great interest, although fecal sample collection methods which impact microbiome studies are sometimes neglected. Here, we expand on previous work in sample optimization, to promote high quality microbiome data. To compare fecal sample collection methods, amplicons from the bacterial 16S rRNA gene (V4) and fungal (ITS2) region, as well as short chain fatty acid (SCFA) concentrations were determined in fecal material over three timepoints. We demonstrated that spot sampling of stool results in variable detection of some microbial members, and inconsistent levels of SCFA; therefore, sample homogenization prior to subsequent analysis or subsampling is recommended. We also identify a trend in microbial and metabolite composition that shifts over two consecutive stool collections less than 25 h apart. Lastly, we show significant differences in bacterial composition that result from collecting stool samples in OMNIgene·Gut tube (DNA Genotec) or Stool Nucleic Acid Collection and Preservation Tube (NORGEN) compared to immediate freezing. To assist with planning fecal sample collection and storage procedures for microbiome investigations with multiple analyses, we recommend participants to collect the first full bowel movement of the day and freeze the sample immediately after collection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.