ArticleParasites & vectors2021
Optimization of the feeding rate of Anopheles farauti s.s. colony mosquitoes in direct membrane feeding assays.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Use of Synthetic Membranes and Bovine Blood in Artificial Feeding Systems of Mosquitoes Improves Feeding Rates: Results from a Systematic Review and Meta-Analysis.The American journal of tropical medicine and hygiene · 2025Pooled it
- Bridging laboratory and field research: Method adjustments to blood feed field-derived Aedes aegypti.PLoS neglected tropical diseases · 2026Article
- Effective Mosquito Repellents: Myrcene- and Cymene-Loaded Nanohydrogels againstPharmaceutics · 2024Article
- Effects of Carbohydrate Intake onInsects · 2024Article
- Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2023Article
- Infectivity of symptomaticCurrent research in parasitology & vector-borne diseases · 2023Article
- Using qPCR to compare the detection ofFrontiers in parasitology · 2023Article
- Asia-Pacific ICEMR: Understanding Malaria Transmission to Accelerate Malaria Elimination in the Asia Pacific Region.The American journal of tropical medicine and hygiene · 2022Article
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- Infectivity of Symptomatic Malaria Patients toFrontiers in cellular and infection microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDirect membrane feeding assays (DMFA) are an important tool to study parasite transmission to mosquitoes. Mosquito feeding rates in these artificial systems require optimization, as there are a number of factors that potentially influence the feeding rates and there are no standardized methods that apply to all anopheline species.
methodsA range of parameters prior to and during direct membrane feeding (DMF) were evaluated for their impact on Anopheles farauti sensu stricto feeding rates, including the starving conditions and duration of starving prior to feeding, membrane type, DMF exposure time, mosquito age, feeding in the light versus the dark, blood volume, mosquito density and temperature of water bath.
resultsThe average successful DMFA feeding rate for An. farauti s.s. colony mosquitoes increased from 50 to 85% when assay parameters were varied. Overnight starvation and Baudruche membrane yielded the highest feeding rates but rates were also affected by blood volume in the feeder and the mosquito density in the feeding cups. Availability of water during the pre-feed starvation period did not significantly impact feeding rates, nor did the exposure duration to blood in membrane feeders, the age of mosquitoes (3, 5 and 7 days post-emergence), feeding in the light versus the dark, or the temperature (34 °C, 38 °C, 42 °C and 46 °C) of the water bath.
conclusionOptimal feeding conditions in An. farauti s.s. DMFA were to offer 50 female mosquitoes in a cup (with a total surface area of ~ 340 cm
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