ArticleReproductive biology and endocrinology : RB&E2021
High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility.
Article in Reproductive biology and endocrinology : RB&E, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Age-Associated Metabolomic Changes in Human Spermatozoa.International journal of molecular sciences · 2026Article
- Exploratory seminal plasma metabolomics and independent HPLC-UV evaluation in men with impaired semen quality.Frontiers in endocrinology · 2026Article
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- Supplementation of Trimethylamine N-Oxide or Betaine in Semen Improves Quality of Boar Spermatozoa Stored at 17 °C Following Hydrostatic Pressure Stress.Life (Basel, Switzerland) · 2025Article
- Sphingolipids and Male Reproductive Health: A Narrative Review of Their Roles in Spermatogenesis, Fertility, and Dysfunction.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Seminal plasma metabolomics and sperm lipidomics profiles of bull semen with different total progressive motile sperm count.Journal of animal science · 2025Article
- Effect of Seminal Plasma on the Freezability of Boar Sperm.Animals : an open access journal from MDPI · 2024Article
- A Mendelian randomization study on causal relationship between metabolic factors and abnormal spermatozoa.Translational andrology and urology · 2024Article
- Broiler breeder putative lipid biomarkers associated with sperm mobility.Frontiers in physiology · 2024Article
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- Lipidomics profiles of human spermatozoa: insights into capacitation and acrosome reaction using UPLC-MS-based approach.Frontiers in endocrinology · 2023Article
- Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility.Frontiers in pharmacology · 2023Article
- Relative Abundance of Lipid Metabolites in Spermatozoa across Three Compartments.International journal of molecular sciences · 2022Article
- Metabolomics of Human Semen: A Review of Different Analytical Methods to Unravel Biomarkers for Male Fertility Disorders.International journal of molecular sciences · 2022Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIt has been previously demonstrated that cholesterol content and cholesterol/phospholipid ratio were significantly higher in asthenozoospermia and oligoasthenoteratozoospermia. The majority of published studies have investigated the fatty acid composition of phospholipids rather than lipids themselves. This study evaluated the lipid composition of asthenozoospermic and normozoospermic spermatozoa, and identified the exact lipid species that correlated with sperm motility.
methodsA total of 12 infertile asthenozoospermia patients and 12 normozoospermia subjects with normal sperm motility values were tested for semen volume, sperm concentration, count, motility, vitality and morphology. High-coverage targeted lipidomics with 25 individual lipid classes was performed to analyze the sperm lipid components and establish the exact lipid species that correlated with sperm motility.
resultsA total of 25 individual lipid classes and 479 lipid molecular species were identified and quantified. Asthenozoospermic spermatozoa showed an increase in the level of four lipid classes, including Cho, PE, LPI and GM3. A total of 48 lipid molecular species were significantly altered between normozoospermic and asthenozoospermic spermatozoa. Furthermore, the levels of total GM3 and six GM3 molecular species, which were altered in normozoospermic spermatozoa versus asthenozoospermic spermatozoa, were inversely correlated with sperm progressive and total motility.
conclusionsSeveral unique lipid classes and lipid molecular species were significantly altered between asthenozoospermic and normozoospermic spermatozoa, revealing new possibilities for further mechanistic pursuits and highlighting the development needs of culture medium formulations to improve sperm motility.
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