Evidence map›Paper›PMID 34233356›Full record

ReviewPlant & cell physiology2021

Advances in Synthetic Fluorescent Probe Labeling for Live-Cell Imaging in Plants.

Noriyoshi Yagi, Akira Yoshinari, Ryu J Iwatate, Reika Isoda, Wolf B Frommer, Masayoshi Nakamura

Abstract readReview
In one paragraph

Review in Plant & cell physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Review
  3. Isolation and Transfection of Nicotiana benthamiana Mesophyll Protoplasts.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  4. Article
  5. Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024
    Review
  6. A novel toolbox to record CLE peptide signaling.Frontiers in plant science · 2024
    Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Noriyoshi YagiInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Akira YoshinariInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Ryu J IwatateInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.ORCID 0000-0001-6111-7809
Reika IsodaInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Wolf B FrommerInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Masayoshi NakamuraInstitute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya 464-8601, Japan.ORCID 0000-0002-7107-4942

Funding

Deutsche Forschungsgemeinschaft EXC-2048/1 project ID 390686111Human Frontier Science Program CDAJapan Society for the Promotion of Science 19H000932Japan Society for the Promotion of Science 19K16164Japan Society for the Promotion of Science 20K21424the Alexander von Humboldt Professorship
6 · The paper itself

Abstract

Fluorescent probes are powerful tools for visualizing cellular and subcellular structures, their dynamics and cellular molecules in living cells and enable us to monitor cellular processes in a spatiotemporal manner within complex and crowded systems. In addition to popular fluorescent proteins, a wide variety of small-molecule dyes have been synthesized through close association with the interdisciplinary field of chemistry and biology, ranging from those suitable for labeling cellular compartments such as organelles to those for labeling intracellular biochemical and biophysical processes and signaling. In recent years, self-labeling technologies including the SNAP-tag system have allowed us to attach these dyes to cellular domains or specific proteins and are beginning to be employed in plant studies. In this mini review, we will discuss the current range of synthetic fluorescent probes that have been exploited for live-cell imaging and the recent advances in the application that enable genetical tagging of synthetic probes in plant research.

Indexed as

Fluorescent DyesImaging, Three-DimensionalIntravital MicroscopyMicroscopy, FluorescencePlant CellsFluorescent DyesFluorescent probes •Live-cell imaging •Self-labeling protein tag •SNAP-tag •Synthetic dyes

Identifiers

PMID34233356
PMCPMC8579277

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.