Evidence map›Paper›PMID 34232061›Full record

ArticleApplied and environmental microbiology2021

RecT Recombinase Expression Enables Efficient Gene Editing in

Victor Chen, Matthew E Griffin, Pascal Maguin, Andrew Varble, Howard C Hang

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Characterization ofVeterinary sciences · 2026
    Article
  6. Review
  7. CRISPR-Cas systems in enterococci.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
    Review
  8. Review
  9. Review
  10. Article
  11. Secreted antigen A peptidoglycan hydrolase is essential forbioRxiv : the preprint server for biology · 2024
    Article
  12. Article
  13. Article
  14. Recombineering in Non-Model Bacteria.Current protocols · 2022
    Article
  15. Article
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Victor ChenLaboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller Universitygrid.134907.8, New York, New York, USA.
Matthew E GriffinLaboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller Universitygrid.134907.8, New York, New York, USA.
Pascal MaguinLaboratory of Bacteriology, The Rockefeller Universitygrid.134907.8, New York, New York, USA.
Andrew VarbleLaboratory of Bacteriology, The Rockefeller Universitygrid.134907.8, New York, New York, USA.
Howard C HangLaboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller Universitygrid.134907.8, New York, New York, USA.
Rockefeller University · USScripps Institution of Oceanography · US

Funding

Studies on Protein LipidationR01GM087544 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI HANG, HOWARD C · 2010 to 2023
$4.4M
Immunity and Infectious DiseaseT32AI070084 · NIAID · ROCKEFELLER UNIVERSITY · PI Michel C Nussenzweig · 2008 to 2026
$3.3M
Elucidation of Commensal Bacteria Mechanisms Required for Host ProtectionR01GM103593 · NIGMS · ROCKEFELLER UNIVERSITY · PI HANG, HOWARD C · 2013 to 2020
$2.8M
Translation of commensal bacteria mechanism for immunotherapyR01CA245292 · NCI · SCRIPPS RESEARCH INSTITUTE, THE · PI HANG, HOWARD C · 2020 to 2024
$2.7M
Distal gut microbiome targets of host anti-proteolytic proteins during colitisR21AI156674 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI HANG, HOWARD C · 2021 to 2022
$488k
Division of Intramural Research, National Institute of Allergy and Infectious Diseases (DIR, NIAID) 1R21AI156674-01HHS | NIH | National Cancer Institute (NCI) 5R01CA245292-02HHS | NIH | National Institute of General Medical Sciences (NIGMS) 2R01GM087544-10NCI NIH HHS R01 CA245292NIAID NIH HHS R21 AI156674NIAID NIH HHS T32 AI070084NIGMS NIH HHS R01 GM087544NIGMS NIH HHS R01 GM103593
6 · The paper itself

Abstract

Enterococcus faecium is a ubiquitous Gram-positive bacterium that has been recovered from the environment, food, and microbiota of mammals. Commensal strains of E. faecium can confer beneficial effects on host physiology and immunity, but antibiotic usage has afforded antibiotic-resistant and pathogenic isolates from livestock and humans. However, the dissection of E. faecium functions and mechanisms has been restricted by inefficient gene-editing methods. To address these limitations, here, we report that the expression of E. faecium RecT recombinase significantly improves the efficiency of recombineering technologies in both commensal and antibiotic-resistant strains of E. faecium and other

Indexed as

Gene EditingBacterial ProteinsCRISPR-Associated Protein 9CRISPR-Cas SystemsEnterococcus faeciumRecombinasesStreptococcus pyogenesBacterial ProteinsCRISPR-Associated Protein 9RecombinasesbiotechnologyCRISPRE. faeciumEnterococcusgeneticsrecombineeringVRE

Identifiers

PMID34232061
PMCPMC8388837
OpenAlexW3180910753

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.