Evidence map›Paper›PMID 34231932›Full record

ArticleCell proliferation2021

Dental pulp stem cell-derived exosomes alleviate cerebral ischaemia-reperfusion injury through suppressing inflammatory response.

Song Li, Lihua Luo, Yan He, Ruohan Li, Yangfan Xiang, Zhenjie Xing, Yejian Li, Abdullkhaleg Ali Albashari, Xiangyan Liao, Keke Zhang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
67citing papers in PubMed, 5 pooled it
6.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

67 citing papers in PubMed, 5 syntheses or guidelines pooled it, 107 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Pooled it
  5. Exosomes in pathogenesis, diagnosis, and therapy of ischemic stroke.Frontiers in bioengineering and biotechnology · 2022
    Pooled it
  6. Article
  7. Article
  8. Fucoidan Oligosaccharides fromMarine drugs · 2026
    Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Review
  15. Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025
    Review
  16. Article
  17. [Recent advances on the role of exosomes in neurodegenerative diseases].Se pu = Chinese journal of chromatography · 2025
    Review
  18. Review
  19. Review
  20. Article

7 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Song LiDepartment of Neurosurgery, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.
Lihua LuoSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.ORCID https://orcid.org/0000-0002-3017-5165
Yan HeLab of Regenerative Medicine, Tianyou Hospital, Wuhan University, of Science and Technology, Wuhan, China.ORCID https://orcid.org/0000-0002-0949-8525
Ruohan LiCentre of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, China.
Yangfan XiangSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Zhenjie XingSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Yejian LiSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Abdullkhaleg Ali AlbashariSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Xiangyan LiaoSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Keke ZhangSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.
Liang GaoDepartment of Shanghai Tenth People's Hospital Clinical Medical College, Nanjing Medical University, Nanjing, China.ORCID https://orcid.org/0000-0002-5184-1235
Qingsong YeSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.ORCID https://orcid.org/0000-0002-2868-9247
Wenzhou Medical University · CNTongji University · CNWuhan University · CNWuhan University of Science and Technology · CN

Funding

Shanghai Hospital Development Center SHDC12017X17the National Natural Science Funding of China 81871503the Wenzhou Major Scientific and Technological Innovation Key Medical and Health Project ZY2019010Zhejiang Provincial Natural Science Foundation of China LGF21H140007
6 · The paper itself

Abstract

objectivesThe study aimed to determine whether dental pulp stem cell-derived exosomes (DPSC-Exos) exert protective effects against cerebral ischaemia-reperfusion (I/R) injury and explore its underlying mechanism. MATERIALS AND

methodsExosomes were isolated from the culture medium of human DPSC. Adult male C57BL/6 mice were subjected to 2 hours transient middle cerebral artery occlusion (tMCAO) injury followed by 2 hours reperfusion, after which singular injection of DPSC-Exos via tail vein was administrated. Brain oedema, cerebral infarction and neurological impairment were measured on day 7 after exosomes injection. Then, oxygen-glucose deprivation-reperfusion (OGD/R) induced BV2 cells were studied to analyse the therapeutic effects of DPSC-Exos on I/R injury in vitro. Protein levels of TLR4, MyD88, NF-κB p65, HMGB1, IL-6, IL-1β and TNF-α were determined by western blot or enzyme-linked immunosorbent assay. The cytoplasmic translocation of HMGB1 was detected by immunofluorescence staining.

resultsDPSC-Exos alleviated brain oedema, cerebral infarction and neurological impairment in I/R mice. DPSC-Exos inhibited the I/R-mediated expression of TLR4, MyD88 and NF-κB significantly. DPSC-Exos also reduced the protein expression of IL-6, IL-1β and TNF-α compared with those of the control both in vitro and in vivo. Meanwhile, DPSC-Exos markedly decreased the HMGB1 cytoplasmic translocation induced by I/R damage.

conclusionsDPSC-Exos can ameliorate I/R-induced cerebral injury in mice. Its anti-inflammatory mechanism might be related with the inhibition of the HMGB1/TLR4/MyD88/NF-κB pathway.

Indexed as

AnimalsCell SurvivalCytokinesCytoplasmDental PulpDisease Models, AnimalExosomesHMGB1 ProteinInflammationInterleukin-1betaInterleukin-6MaleMiceMice, Inbred C57BLMicrogliaMyeloid Differentiation Factor 88CytokinesHMGB1 ProteinInterleukin-1betaInterleukin-6Myd88 protein, mouseMyeloid Differentiation Factor 88Toll-Like Receptor 4Transcription Factor RelAcerebral ischaemia-reperfusion injurydental pulp stem cellsexosomesneuroinflammationoxygen-glucose deprivation-reperfusion

Identifiers

PMID34231932
PMCPMC8349657
OpenAlexW3180590157

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.