ArticleJournal of animal science and biotechnology2021
Interaction between early in ovo stimulation of the gut microbiota and chicken host - splenic changes in gene expression and methylation.
Article in Journal of animal science and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Methylation and expression dynamics in broiler liver followingJournal of veterinary research · 2026Article
- Host genetic and environmental determinants of chicken gut microbiota: A review.Poultry science · 2026Review
- Effects of in ovo stimulation with potential epigenetic modulators on immune system phenotype across three generations in a chicken model.Scientific reports · 2025Article
- Changes in the gene expression and methylation in chicken cecal tonsils after in ovo administration of bioactive substances.Scientific reports · 2023Article
- MicroRNA expression in immune tissues of adult chickens after embryo stimulation with bioactive substances.Scientific reports · 2023Article
- Physiological effects ofFrontiers in veterinary science · 2023Review
- Pre-hatching and post-hatching environmental factors related to epigenetic mechanisms in poultry.Journal of animal science · 2022Review
- Epigenetic changes in poultry due to reprogramming of the gut microbiota.Animal frontiers : the review magazine of animal agriculture · 2021Article
- Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes.Animals : an open access journal from MDPI · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundEpigenetic regulation of the gene expression results from interaction between the external environment and transcription of the genetic information encoded in DNA. Methylated CpG regions within the gene promoters lead to silencing of the gene expression in most cases. Factors contributing to epigenetic regulation include intestinal microbiota, which in chicken can be potently modified by in ovo stimulation. The main aim of this study was to determine global and specific methylation patterns of the spleen under the influence of host-microbiome interaction.
resultsFertilized eggs of two genotypes: Ross 308 and Green-legged Partridgelike were in ovo stimulated on d 12 of incubation. The injected compounds were as follows: probiotic - Lactococcus lactis subsp. cremoris IBB477, prebiotic - galactooligosaccharides, and synbiotic - combination of both. Chickens were sacrificed on d 42 post-hatching. Spleen was collected, RNA and DNA were isolated and intended to gene expression, gene methylation and global methylation analysis. We have proved that negative regulation of gene expression after administration of bioactive substances in ovo might have epigenetic character. Epigenetic changes depend on the genotype and the substance administered in ovo.
conclusionEpigenetic nature of microbial reprogramming in poultry and extension of issues related to host-microbiome interaction is a new direction of this research.
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