ArticleACS nano2021
Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement.
Article in ACS nano, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.ACS sensors · 2026Article
- Suppression of Aggregation Enhances Placement Fidelity of Asymmetrical Right Triangle DNA Origami.JACS Au · 2026Article
- Single-Molecule Detection Concepts Enabled by DNA Origami.Micromachines · 2026Review
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- Advances in Strategies for Colloidal Self-Assembly.Chemical reviews · 2026Review
- Light-Guided Molecular Patterning for High-Throughput Single-Molecule Mechanical Characterization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Deterministic quantum light emitters in DNA origami-engineered molecule-MoS₂ hybrids.Light, science & applications · 2026Article
- Tunable DNA Origami Nanosensors for Detection of Multiscale Spatial Ion Concentration Gradients.bioRxiv : the preprint server for biology · 2025Article
- Bringing Attomolar Detection to the Point-of-Care with Nanopatterned DNA Origami Nanoantennas.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Plasmonic DNA-Barcoded Virion Nano-Oscillators for Multiplexed Quantification of Small-Molecule Binding Kinetics to Membrane Proteins.Angewandte Chemie (International ed. in English) · 2025Article
- Vertical DNA Nanostructure Arrays: Facilitating Functionalization on Macro-Scale Surfaces.ACS nano · 2025Article
- Macroscale-area patterning of three-dimensional DNA-programmable frameworks.Nature communications · 2025Article
- Nanopatterned Monolayers of Bioinspired, Sequence-Defined Polypeptoid Brushes for Semiconductor/Bio Interfaces.ACS nano · 2024Article
- Site-directed placement of three-dimensional DNA origami.Nature nanotechnology · 2023Article
- DNA nanopores as artificial membrane channels for bioprotonics.Nature communications · 2023Article
- Recent Advances in DNA Origami-Engineered Nanomaterials and Applications.Chemical reviews · 2023Review
- DNA as grabbers and steerers of quantum emitters.Nanophotonics (Berlin, Germany) · 2023Review
- Advancing the Utility of DNA Origami Technique through Enhanced Stability of DNA-Origami-Based Assemblies.Bioconjugate chemistry · 2023Review
- Harnessing DNA Nanotechnology and Chemistry for Applications in Photonics and Electronics.Bioconjugate chemistry · 2023Review
- A single strand: A simplified approach to DNA origami.Frontiers in chemistry · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA origami placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a ∼100 nm self-assembled template for single-molecule organization with 5 nm resolution and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly trained personnel, making it prohibitively expensive for researchers. Here, we introduce a cleanroom-free, $1 benchtop technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, 2-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.